Silicon substrate as a novel cell culture device for myoblast cells

Journal of Biomedical Science

Tzu-Liang Bill Tseng⁴  Kym Fraser³  Jorge I Rodriguez-Devora²  Mohammod K Bhuyan¹ 

[1] Department of Mechanical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA;Department of Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA;Centre for Logistics, Aalborg University, Aalborg, Denmark;Department of Industrial, Manufacturing and System Engineering, University of Texas at El Paso, El Paso, TX 79968, USA

关键词: Cell carrier;    Silicon substrate;    Photovoltaic effect;    Cell culturing;   

Others  :  817523
DOI  :  10.1186/1423-0127-21-47

 received in 2014-02-21, accepted in 2014-05-05,  发布年份 2014

【 摘 要 】

Background

Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy.

Results

It was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate.

Conclusions

This study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.

【 授权许可】

   
2014 Bhuyan et al.; licensee BioMed Central Ltd.

【 图 表 】

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