Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes

Lipids in Health and Disease

Hamden Khaled²  Saloua Lassoued¹  Abdelfetteh Elfekih²  Fatma Ben Abdallah¹  Riadh Ben Mansour¹  Bochra Gargouri¹ 

[1] Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, 3038, Tunisie;Unité de Physiologie Animale, Faculté des Sciences de Sfax, 3037, Tunisie

关键词: lymphocytes;    catalase;    superoxide dismutase;    thiol;    malondialdehyde;    Dimethoate;    Quercetin;   

Others  :  1212534
DOI  :  10.1186/1476-511X-10-149

 received in 2011-06-03, accepted in 2011-08-23,  发布年份 2011

【 摘 要 】

Background

The aim of this study is to investigate the effect of quercetin in alleviating the cytotoxic effects of Dimethoate in human peripheral blood lymphocytes.

Methods

Lymphocytes were divided into too groups. The first group, lymphocytes were incubated for 4 h at 37°C with different concentrations (0, 40, 60 and 100 mM) of Dimethoate. The second group was preincubated with quercetin for 30 min and followed by Dim incubation for 4 h at 37°C.

Results

Following in vitro incubation, Dimethoate caused a significant increase in malondialdehyde levels, a significant decrease in thiol levels, as well as a significant increase in superoxide dismutase, and catalase activities in lymphocytes at different concentrations. Quercetin pretreated lymphocytes showed a significant protection against the cytotoxic effects inducted by Dimethoate on the studied parameters.

Conclusion

In conclusion, antioxidant quercetin could protect against Dimethoate-induced oxidative stress by decreasing lipid peroxidation, protein oxidation and increasing superoxide dismutase and catalase activities in human lymphocytes.

【 授权许可】

   
2011 Gargouri et al; licensee BioMed Central Ltd.

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