Serum response factor modulates neuron survival during peripheral axon injury

Journal of Neuroinflammation

Bernd Knöll¹  Daniela Sinske¹  Sina Stern² 

[1] Current addresses: Institute for Physiological Chemistry, Ulm University, Ulm, 89081, Germany;Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Bonn, 53175, Germany

关键词: Microglia;    Axon;    SRF;    Regeneration;    Motorneuron;    Immune cell;    Facial nerve;   

Others  :  1212654
DOI  :  10.1186/1742-2094-9-78

 received in 2011-08-15, accepted in 2012-04-26,  发布年份 2012

【 摘 要 】

Background

The transcription factor SRF (serum response factor) mediates neuronal survival in vitro. However, data available so far suggest that SRF is largely dispensable for neuron survival during physiological brain function.

Findings

Here, we demonstrate that upon neuronal injury, that is facial nerve transection, constitutively-active SRF-VP16 enhances motorneuron survival. SRF-VP16 suppressed active caspase 3 abundance in vitro and enhanced neuron survival upon camptothecin induced apoptosis. Following nerve fiber injury in vitro, SRF-VP16 improved survival of neurons and re-growth of severed neurites. Further, SRF-VP16 enhanced immune responses (that is microglia and T cell activation) associated with neuronal injury in vivo. Genome-wide transcriptomics identified target genes associated with axonal injury and modulated by SRF-VP16.

Conclusion

In sum, this is a first report describing a neuronal injury-related survival function for SRF.

【 授权许可】

   
2012 Stern et al.; licensee BioMed Central Ltd.

【 图 表 】

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