Assessment of sensitization to grape and wine allergens as possible causes of adverse reactions to wine: a pilot study

Clinical and Translational Allergy

Heinz Decker¹  Joachim Saloga²  Bärbel Heydenreich²  Petra Fronk¹  Iris Bellinghausen²  Nadine Jaeckels¹ 

[1] Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob-Welder-Weg 26, Mainz, D-55128, Germany;Department of Dermatology, University Medical Center Mainz, Langenbeckstraße 1, Mainz, D-55131, Germany

关键词: CCD;    CAST;    Wine;    LTP;    Wine intolerance;   

Others  :  1219069
DOI  :  10.1186/s13601-015-0065-8

 received in 2015-03-17, accepted in 2015-05-20,  发布年份 2015

【 摘 要 】

Background

In a recently performed survey with 4000 randomly selected persons, 68 (7.2 %) of 948 respondents reported intolerance and/or allergy-like symptoms to wine. The aim of this study was to analyze whether a real sensitization to wine proteins could be confirmed by diagnostic and/or immunological settings.

Findings

For this purpose, 19 subjects with self-reported intolerance to wine of the invited subjects and 10 controls without a history of intolerance participated in an allergological examination (skin prick test, ImmunoCAP for determination of specific IgE antibodies, CAST for testing basophil activation, ImmunoBlot for testing specificity of IgE-antibodies). For the allergological work-up red and white grapes, selected wines, and the purified lipid transfer protein (LTP), a known grape allergen, were used. 7 subjects showed evidence of IgE sensitization to wine or grape extracts, including one control. One participant with symptoms of intolerance showed a positive skin prick test to red grape, a positive ImmunoCAP to grape, a positive cellular antigen stimulation test (CAST) and inhibition of Western blot by removal of cross-reactive carbohydrate determinants (CCD).

Conclusion

The presented study focused on the grape protein-related IgE-mediated cause of intolerance to wine (true allergy) and not on other wine components or fining agents (other forms of intolerance). A sensitization to grape and wine proteins was observed in our cohort. In one case, this reactivity could be explained by cross-reactivity to CCD. The results of this pilot study need to be validated in greater cohorts.

【 授权许可】

   
2015 Jaeckels et al.; licensee BioMed Central.

【 图 表 】

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【 参考文献 】

• [1]German JB, Walzem RL: The health benefits of wine. Annu Rev Nutr 2000, 20:561-593.
• [2]Pastorello EA, Farioli L, Pravettoni V, Ortolani C, Fortunato D, Giuffrida MG, et al.: Identification of grape and wine allergens as an endochitinase 4, a lipid-transfer protein, and a thaumatin. J Allergy Clin Immunol 2003, 111:350-359.
• [3]Vassilopoulou E, Zuidmeer L, Akkerdaas J, Tassios I, Rigby NR, Mills EN, et al.: Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome. Int Arch Allergy Immunol 2007, 143:92-102.
• [4]Giannoccaro F, Munno G, Riva G, Pugliese S, Paradiso MT, Ferrannini A: Oral allergy syndrome to grapes. Allergy 1998, 53:451-452.
• [5]Senna G, Mistrello G, Roncarolo D, Crivellaro M, Bonadonna P, Schiappoli M, et al.: Exercise-induced anaphylaxis to grape. Allergy 2001, 56:1235-1236.
• [6]Rodriguez A, Trujillo MJ, Matheu V, Baeza ML, Zapatero L, Martinez M: Allergy to grape: A case report. Pediatr Allergy Immunol 2001, 12:289-290.
• [7]Borras EA, Faraudo EB, Jane PG, Zavala BB: Alcohol-induced anaphylaxis to grape. Allergol Immunopathol (Madr) 2007, 35:159-161.
• [8]Schad SG, Trcka J, Vieths S, Scheurer S, Conti A, Brocker EB, et al.: Wine anaphylaxis in a German patient: IgE-mediated allergy against a lipid transfer protein of grapes. Int Arch Allergy Immunol 2005, 136:159-164.
• [9]Schad SG, Trcka J, Lauer I, Scheurer S, Trautmann A: Wine allergy in a wine-growing district: tolerance induction in a patient with allergy to grape lipid-transfer protein. World Allergy Organ J 2010, 3:1-5.
• [10]Sbornik M, Rakoski J, Mempel M, Ollert M, Ring J: IgE-mediated type-I-allergy against red wine and grapes. Allergy 2007, 62:1339-1340.
• [11]Jaeckels N, Tenzer S, Rosfa S, Schild HJ, Decker H, Wigand P: Purification and structural characterisation of lipid transfer protein from red wine and grapes. Food Chem 2013, 138:263-269.
• [12]Wigand P, Blettner M, Saloga J, Decker H: Prevalence of wine intolerance: results of a survey from Mainz, Germany. Dtsch Arztebl Int 2012, 109:437-444.
• [13]Aberer W, Holzweber F, Hemmer W, Koch L, Bokanovic D, Fellner W, et al.: Inhibition of cross-reactive carbohydrate determinants (CCDs) enhances the selectivity of in vitro allergy diagnosis. Allergologie 2014, 37:46-53.
• [14]Vieths S, Scheurer S, Ballmer-Weber B: Current understanding of cross-reactivity of food allergens and pollen. Ann N Y Acad Sci 2002, 964:47-68.
• [15]Gonzalez-Quintela A, Gomez-Rial J, Valcarcel C, Campos J, Sanz ML, Linneberg A, et al.: Immunoglobulin-E reactivity to wine glycoproteins in heavy drinkers. Alcohol 2011, 45:113-122.
• [16]Maintz L, Novak N: Histamine and histamine intolerance. Am J Clin Nutr 2007, 85:1185-1196.
• [17]Vally H, de Klerk N, Thompson PJ: Alcoholic drinks: important triggers for asthma. J Allergy Clin Immunol 2000, 105:462-467.
• [18]Bodmer S, Imark C, Kneubuhl M: Biogenic amines in foods: histamine and food processing. Inflamm Res 1999, 48:296-300.
• [19]Tawakol A, Omland T, Creager MA: Direct effect of ethanol on human vascular function. Am J Physiol Heart Circ Physiol 2004, 286:H2468-H2473.
• [20]Nakagawa Y, Sumikawa Y, Nakamura T, Itami S, Katayama I, Aoki T: Urticarial reaction caused by ethanol. Allergol Int 2006, 55:411-414.
• [21]Vassilopoulou E, Karathanos A, Siragakis G, Giavi S, Sinaniotis A, Douladiris N, et al.: Risk of allergic reactions to wine, in milk, egg and fish-allergic patients. Clin Transl Allergy 2011, 1:10. BioMed Central Full Text
• [22]Rolland JM, Apostolou E, Deckert K, de Leon MP, Douglass JA, Glaspole IN, et al.: Potential food allergens in wine: double-blind, placebo-controlled trial and basophil activation analysis. Nutrition 2006, 22:882-888.
• [23]Boban M, Modun D, Music I, Vukovic J, Brizic I, Salamunic I, et al.: Red wine induced modulation of vascular function: Separating the role of polyphenols, ethanol, and urates. J Cardiovasc Pharmacol 2006, 47:695-701.