Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice

European Journal of Medical Research

S Langer³  H Homann³  A Stachon²  A Daigeler³  C Andree⁴  HU Steinau³  P Babilas¹  H Lilienfein³  MH Kirschner⁵  O Goertz³ 

[1] Department of Dermatology, Regensburg University Medical Center, Germany;Institute of Clinical Chemistry, Transfusion, and Laboratory Medicine, Ruhr-University Bochum, Germany;Department of Plastic and Hand Surgery, Burn Center, University Hospital Bergmannsheil, Ruhr-University Bochum, Germany;Sana Hospital, Düsseldorf, Germany;BBD Aesculap GmbH, Tuttlingen, Germany

关键词: hemodilution;    intracardiac access;    free hemoglobin;    oxygen carrier;    intravital fluorescent microscopy;    hairless mice;    MP4;   

Others  :  1093917
DOI  :  10.1186/2047-783X-14-3-123

 received in 2008-12-19, accepted in 2009-01-12,  发布年份 2009

【 摘 要 】

Objective

Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfusion is the current standard of treatment with its attendant risks. The aim of this study was to investigate the potential of the chemically modified hemoglobin, MP4, to reduce skin flap necrosis and its effect on selected blood markers and kidneys.

Materials and methods

Tissue ischemia was induced in the ear of hairless mice (n = 26). Hemodilution was performed by replacing one third of blood volume with the similar amount of MP4, dextran, or blood. The extent of non-perfused tissue was assessed by intravital fluorescent microscopy.

Results

Of all groups, MP4 showed the smallest area of no perfusion (in percentage of the ear ± SEM: 16.3% ± 2.4), the control group the largest (22.4% ± 3.5). Leukocytes showed a significant increase in the MP4 and dextran group (from 8.7 to 13.6 respectively 15.4*10⁹/l). On histology no changes of the kidneys could be observed.

Conclusion

MP4 causes an increase of leukocytes, improves the oxygen supply of the tissue and shows no evidence of renal impairment.

【 授权许可】

   
2009 I. Holzapfel Publishers

【 图 表 】

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