φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. mallei strain infectivity

BMC Microbiology

Herbert P Schweizer²  Kent J Voorhees⁴  Shannon L Johnson³  David DeShazer¹  Christopher R Cox⁴  Brian H Kvitko² 

[1] United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA;Department of Microbiology, Immunology and Pathology, Colorado State University, IDRC at Foothills Campus, Fort Collins, CO, 80523-0922, USA;Los Alamos National Laboratory Genome Science Group, Joint Genomics Institute, Los Alamos, NM, USA;Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO, USA

关键词: Phage-based diagnostics;    Prophage distribution;    P2;    B. mallei;    Burkholderia pseudomallei;    Bacteriophage;   

Others  :  1144732
DOI  :  10.1186/1471-2180-12-289

 received in 2012-07-24, accepted in 2012-11-28,  发布年份 2012

【 摘 要 】

Background

Burkholderia pseudomallei and B. mallei are closely related Category B Select Agents of bioterrorism and the causative agents of the diseases melioidosis and glanders, respectively. Rapid phage-based diagnostic tools would greatly benefit early recognition and treatment of these diseases. There is extensive strain-to-strain variation in B. pseudomallei genome content due in part to the presence or absence of integrated prophages. Several phages have previously been isolated from B. pseudomallei lysogens, for example φK96243, φ1026b and φ52237.

Results

We have isolated a P2-like bacteriophage, φX216, which infects 78% of all B. pseudomallei strains tested. φX216 also infects B. mallei, but not other Burkholderia species, including the closely related B. thailandensis and B. oklahomensis. The nature of the φX216 host receptor remains unclear but evidence indicates that in B. mallei φX216 uses lipopolysaccharide O-antigen but a different receptor in B. pseudomallei. The 37,637 bp genome of φX216 encodes 47 predicted open reading frames and shares 99.8% pairwise identity and an identical strain host range with bacteriophage φ52237. Closely related P2-like prophages appear to be widely distributed among B. pseudomallei strains but both φX216 and φ52237 readily infect prophage carrying strains.

Conclusions

The broad strain infectivity and high specificity for B. pseudomallei and B. mallei indicate that φX216 will provide a good platform for the development of phage-based diagnostics for these bacteria.

【 授权许可】

   
2012 Kvitko et al.; licensee BioMed Central Ltd.

【 图 表 】

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