Anti-diabetic, anti-oxidant and anti-hyperlipidemic activities of Melastoma malabathricum Linn. leaves in streptozotocin induced diabetic rats

BMC Complementary and Alternative Medicine

Mohd Mujeeb¹  Firoz Anwar²  Pushpraj S Gupta³  Danish Ahmed³  Vikas Kumar³ 

[1] Department of Photochemistry & Pharmacognosy, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India;Sidharatha Institute of Pharmacy, Dehradun, Uttarakhand 248001, India;Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS)-Deemed University, Allahabad, Uttar Pradesh 211007, India

关键词: Glibenclamide;    Antihyperlipidemic;    Antidiabetic;    Streptozotocin;    Melastoma malabathricum;   

Others  :  1221010
DOI  :  10.1186/1472-6882-13-222

 received in 2013-05-15, accepted in 2013-09-04,  发布年份 2013

【 摘 要 】

Background

Melastoma malabathricum (MM) Linn leaves traditionally use in the treatment of diabetic conditions. The aim of the present investigation was to evaluate the antioxidant, antihyperlipidemic and antidiabetic activity of methanolic extract taken from Melastoma malabathricum Linn (Melastomaceae).

Methods

The methanolic leaves extract of MM Linn leaves used for the study. Chemical test of different extract, acute toxicity study and oral glucose test was performed. Diabetes was induced in rat by single intra-peritoneal injection of streptozotocin (55 mg/kg). The rats were divided into following groups: Group I – normal control, Group II (Vehicle) – diabetic control, Group III (STZ-toxic) – MM I (100 mg/kg, p.o.), Group IV – MM II (250 mg/kg, p.o.), Group V – MM III (500 mg/kg, p.o.), Group VI – glibenclamide (10 mg/kg, p.o.). Bodyweight of each rat in the different groups was recorded daily. Biochemical and antioxidant enzyme parameters were determined on day 28. Histology of different organ (heart, liver, kidney, and pancreas) was performed after sacrificing the rats with euthanasia.

Results

The methanolic extract of MM did not show any acute toxicity up-to the dose of 2000 mg/kg and shown better glucose utilization in oral glucose tolerance test. Orally treatment of different doses of MM leaves extract decreased the level of serum glucose, glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate and increased the level of plasma insulin, hexokinase. MM treatment decreased liver malondialdehyde but increased the level of superoxide dismutase, catalase and glutathione peroxidase. In oral glucose tolerance test observed increased utilization of glucose. Streptozotocin induced diabetes groups rat treated with different doses of MM leaves extract and glibenclamide significantly increased the body weight. Histopathology analysis on different organ of STZ (streptozotocin) induced diabetic rat show there regenerative effect on the liver, kidney, heart and pancreas.

Conclusion

The antioxidant, antihyperlipidemic and antidiabetic effect of methanolic extract from Melastoma malabathricum Linn suggests a potential therapeutic treatment to antidiabetic conditions.

【 授权许可】

   
2013 Kumar et al.; licensee BioMed Central Ltd.

【 图 表 】

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