Pleurotus ostreatus opposes mitochondrial dysfunction and oxidative stress in acetaminophen-induced hepato-renal injury

BMC Complementary and Alternative Medicine

Mohamed F Salem³  Rehab M Samaka²  Rania M Azmy¹  Yahya M Naguib⁴ 

[1]Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Menoufia, Egypt

[2]Department of Pathology, Faculty of Medicine, Menoufia University, Menoufia, Egypt

[3]Genetic Engineering and Biotechnology Research Institute, Sadat City University, Menoufia, Egypt

[4]Department of Clinical Physiology, Faculty of Medicine, Menoufia University, Menoufia, Egypt

关键词: Antioxidant;    Acetaminophen;    Mitochondrial dysfunction;    Acute hepato-renal injury;    Oxidative stress;    Pleurotus ostreatus;   

Others  :  1084772
DOI  :  10.1186/1472-6882-14-494

 received in 2014-03-10, accepted in 2014-12-10,  发布年份 2014

【 摘 要 】

Background

Acetaminophen (APAP)-induced toxicity is a predominant cause of acute hepatic and renal failure. In both humans and rodents toxicity begins with a reactive metabolite that binds to proteins. This leads to mitochondrial dysfunction and nuclear DNA fragmentation resulting in necrotic cell death. Pleurotus ostreatus (an edible oyster mushroom) is well recognized as a flavourful food, as well as a medicinal supplement. In the present study, we evaluated the role of Pleurotus ostreatus in the protection against APAP-induced hepato-renal toxicity. We also explored the mechanism by which Pleurotus ostreatus exerts its effects.

Methods

Ninety adult male Swiss albino mice were divided into three groups (30 mice/group). Mice were offered normal diet (control and APAP groups), or diet supplemented with 10% Pleurotus ostreatus (APAP + Pleurotus ostreatus) for 10 days. Mice were either treated with vehicle (control group, single intra-peritoneal injection.), or APAP (APAP and APAP + Pleurotus ostreatus groups, single intra-peritoneal injection, 500 mg/kg), 24 hours after the last meal.

Results

APAP increased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) glutamate dehydrogenase (GDH), creatinine, blood urea nitrogen (BUN), urinary kidney injury molecule-1 (KIM-1), and hepatic and renal malondialdehyde (MDA) content. APAP decreased hepatic and renal glutathione (GSH) content, as well as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities. Supplementation with Pleurotus ostreatus significantly reduced APAP-induced elevated levels of ALT, AST, GDH, creatinine, BUN, KIM-1and MDA, while GSH level, and GSH-Px and SOD activities were significantly increased. Our findings were further validated by histopathology; treatment with Pleurotus ostreatus significantly decreased APAP-induced cell necrosis in liver and kidney tissues.

Conclusions

We report here that the antioxidant effect of Pleurotus ostreatus opposes mitochondrial dysfunction and oxidative stress accompanying APAP over-dose, with subsequent clinically beneficial effects on liver and kidney tissues.

【 授权许可】

   
2014 Naguib et al.; licensee BioMed Central Ltd.

【 图 表 】

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