Mapping QTL conferring resistance in maize to gray leaf spot disease caused by Cercospora zeina

BMC Genetics

Alexander A Myburg²  Pangirayi Tongoona¹  Frederik J Kloppers³  Felix Middleton³  Jeanne N Korsman⁴  Maryke Carstens⁴  Dave K Berger⁴ 

[1] African Centre for Crop Improvement, University of KwaZulu-Natal, Pietermaritzburg, South Africa;Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;PANNAR SEED (Pty) Ltd, PO Box 19, Greytown, South Africa;Department of Plant Science, Forestry and Agricultural Biotechnology Institute (FABI), Plant Sciences Complex, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa

关键词: Corn;    Maize;    QTL;    Cercospora;    GLS;    Grey leaf spot;    Gray leaf spot;   

Others  :  866252
DOI  :  10.1186/1471-2156-15-60

 received in 2014-01-27, accepted in 2014-05-15,  发布年份 2014

【 摘 要 】

Background

Gray leaf spot (GLS) is a globally important foliar disease of maize. Cercospora zeina, one of the two fungal species that cause the disease, is prevalent in southern Africa, China, Brazil and the eastern corn belt of the USA. Identification of QTL for GLS resistance in subtropical germplasm is important to support breeding programmes in developing countries where C. zeina limits production of this staple food crop.

Results

A maize RIL population (F7:S6) from a cross between CML444 and SC Malawi was field-tested under GLS disease pressure at five field sites over three seasons in KwaZulu-Natal, South Africa. Thirty QTL identified from eleven field trials (environments) were consolidated to seven QTL for GLS resistance based on their expression in at least two environments and location in the same core maize bins. Four GLS resistance alleles were derived from the more resistant parent CML444 (bin 1.10, 4.08, 9.04/9.05, 10.06/10.07), whereas the remainder were from SC Malawi (bin 6.06/6.07, 7.02/7.03, 9.06). QTLs in bin 4.08 and bin 6.06/6.07 were also detected as joint QTLs, each explained more than 11% of the phenotypic variation, and were identified in four and seven environments, respectively. Common markers were used to allocate GLS QTL from eleven previous studies to bins on the IBM2005 map, and GLS QTL “hotspots” were noted. Bin 4.08 and 7.02/7.03 GLS QTL from this study overlapped with hotspots, whereas the bin 6.06/6.07 and bin 9.06 QTLs appeared to be unique. QTL for flowering time (bin 1.07, 4.09) in this population did not correspond to QTL for GLS resistance.

Conclusions

QTL mapping of a RIL population from the subtropical maize parents CML444 and SC Malawi identified seven QTL for resistance to gray leaf spot disease caused by C. zeina. These QTL together with QTL from eleven studies were allocated to bins on the IBM2005 map to provide a basis for comparison. Hotspots of GLS QTL were identified on chromosomes one, two, four, five and seven, with QTL in the current study overlapping with two of these. Two QTL from this study did not overlap with previously reported QTL.

【 授权许可】

   
2014 Berger et al.; licensee BioMed Central Ltd.

【 图 表 】

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