A comparison of balloon injury models of endovascular lesions in rat arteries

BMC Cardiovascular Disorders

Hero van Urk⁴  Paul Mulder²  Elma J Gussenhoven¹  Wim Sluiter³  Randolph G Statius van Eps⁴  Richard van Hillegersberg⁴  Edward EE Gabeler⁴ 

[1] Dept. of Experimental echocardiology (ICIN), Erasmus MC, Rotterdam, The Netherlands;Dept. of Epidemiology and Biostatistics, the Netherlands Institute for Health Sciences;Dept. of Biochemistry, Erasmus MC, Rotterdam, The Netherlands;Dept. of Surgery, Erasmus MC, Rotterdam, The Netherlands

关键词: restenosis;    remodelling;    rats;    balloon injury;   

Others  :  1088621
DOI  :  10.1186/1471-2261-2-16

 received in 2002-04-08, accepted in 2002-09-27,  发布年份 2002

【 摘 要 】

Background

Balloon injury (BI) of the rat carotid artery (CCA) is widely used to study intimal hyperplasia (IH) and decrease in lumen diameter (LD), but CCA's small diameter impedes the evaluation of endovascular therapies. Therefore, we validated BI in the aorta (AA) and iliac artery (CIA) to compare it with CCA.

Methods

Rats underwent BI or a sham procedure (control). Light microscopic evaluation was performed either directly or at 1, 2, 3, 4 and 16 weeks follow-up. The area of IH and the change in LD (LD at 16 weeks minus LD post BI) were compared.

Results

In the BI-groups the area of IH increased to 0.14 ± 0.08 mm² (CCA), 0.14 ± 0.03 mm² (CIA) and 0.12 ± 0.04 mm² (AA) at 16 weeks (NS). The LD decreased with 0.49 ± 0.07 mm (CCA), compared to 0.22 ± 0.07 mm (CIA) and 0.07 ± 0.10 mm (AA) at 16 weeks (p < 0.05). The constrictive vascular remodelling (CVR = wall circumference loss combined with a decrease in LD) was -0.17 ± 0.05 mm in CIA but absent in CCA and AA. No IH, no decrease in LD and no CVR was seen in the control groups.

Conclusions

BI resulted in: (1.) a decrease in LD in CCA due to IH, (2.) a decrease in LD in CIA due to IH and CVR, (3.) no change in LD in AA, (4.) Comparable IH development in all arteries, (5.) CCA has no vasa vasorum compared to CIA and AA, (6.) The CIA model combines good access for 2 F endovascular catheters with a decrease in LD due to IH and CVR after BI.

【 授权许可】

   
2002 Gabeler et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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