Effect of crude leaf extract of Osyris quadripartita on Plasmodium berghei in Swiss albino mice

BMC Complementary and Alternative Medicine

Hassen Mamo¹  Berhanu Erko²  Mirutse Giday²  Senait Girma¹ 

[1] Department of Microbial, Cellular and Molecular Biology, College of Natural Sciences, Addis Ababa University, Addis Ababa, Ethiopia;Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia

关键词: Ethiopia;    Swiss albino mice;    Plasmodium berghei;    in vivo;    Osyris quadripartita;    Antimalarial activity;   

Others  :  1217075
DOI  :  10.1186/s12906-015-0715-3

 received in 2015-03-06, accepted in 2015-06-09,  发布年份 2015

【 摘 要 】

Background

Continuous emergence of multi-drug-resistant malaria parasites and their rapid spread across the globe warrant urgent search for new anti-malarial chemotherapeutics. Traditional medicinal plants have been the main sources for screening active phytochemicals against malaria. Accordingly, this study was aimed at evaluating the anti-malarial activity of Osyris quadripartita Salzm. Ex Decne., a plant which is used for traditional malaria treatment by local people in different parts of Ethiopia.

Methods

Aqueous, chloroform and methanol crude leaf extracts of the plant have been prepared and tested for acute toxicity and anti-malarial efficacy in Plasmodium berghei (ANKA strain)-infected Swiss albino mice.

Results

At three oral doses of 200, 400 and 600 mg/kg the plant material was safe, chemosuppressive and thus prevented body weight loss, hematological abnormalities and increased mice mean survival time compared to the negative control. The most efficacious extract was that of chloroform which prolonged mean mouse survival past day 11 of infection with all the mice in this group having the highest parasitemia suppression rate (41.3 %, at 600 mg/kg) although parasite clearance was not achieved compared to the standard drug (chloroquine) against the parasite.

Conclusion

The finding supports the traditional use of the plant for the treatment of malaria. However, further confirmatory studies followed by isolation and characterization of the active anti-malarial compound (s) of the plant that is/are responsible for the observed parasite suppression is needed before it is recommended for malaria drug search and discovery.

【 授权许可】

   
2015 Girma et al.

【 参考文献 】

• [1]World Malaria Report 2014. World Health Organization, Geneva, Switzerland; 2014.
• [2]National malaria program monitoring and evaluation plan 2014–2020. federal democratic republic of Ethiopia ministry of health. Federal Ministry of Health, Addis Ababa; 2014.
• [3]Ashley EA, Dhorda M, Fairhurst RM, Amaratunga C, Lim P, Suon S et al.. Spread of artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2014; 371:411-23.
• [4]Global plan for insecticide resistance management in malaria vectors. WHO, Geneva; 2012.
• [5]WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO, Geneva; 2007.
• [6]Ramazani A, Zakeri S, Sardari S, Khodakarim N, Djadidt ND. In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense. Malar J. 2010; 9:124. BioMed Central Full Text
• [7]Verma G, Dua VK, Agarwal DD, Atul PK. Anti-malarial activity of Holarrhena antidysenterica and Viola canescens, plants traditionally used against malaria in the Garhwal region of north-west Himalaya. Malar J. 2011; 10:20. BioMed Central Full Text
• [8]Jansen O, Tits M, Angenot L, Nicolas JP, Mol PD, Nikiema JB et al.. Anti-plasmodial activity of Dicoma tomentosa (Asteraceae) and identification of urospermal A-15- O-acetate as the main active compound. Malar J. 2012; 11:289. BioMed Central Full Text
• [9]Mirutse G, Teklehaymanot T, Animut A, Mekonnen Y. Medicinal plants of the Shinasha, Agew-awi and Amhara peoples in northwest Ethiopia. J Ethnopharmacol. 2007; 110:516-525.
• [10]Mesfin F, Demisse S, Teklehaymanot T. An ethnobotanical study of medicinal plants in Wonago Woreda. SNNPR Ethiop J Ethnobiol Ethnomed. 2009; 5:28. BioMed Central Full Text
• [11]Lulekal E, Kelbessa E, Bekele T, Yineger H. An ethnobotanical study of medicinal plants in Mana Angetu District, southeastern Ethiopia. J Ethnobiol Ethnomed. 2008; 4:10. BioMed Central Full Text
• [12]Lulekal E, Asfaw Z, Kelbessa E, Van Damme P. Ethnomedicinal study of plants used for human ailments in Ankober District, North Shewa Zone. Amhara Region Ethiop J Ethnobiol Ethnomed. 2013; 9:63. BioMed Central Full Text
• [13]Watt JM, Breyer-Brandwijk MG. The medicinal and poisonous plants of southern and eastern Africa. Edinburg and London, E. & S. Livingstone Ltd; 1962.
• [14]Belayneh A, Bussa NF. Ethnomedicinal plants used to treat human ailments in the prehistoric place of Harla and Dengego Valley, eastern Ethiopia. J Ethnobiol Ethnomed. 2014; 10:18. BioMed Central Full Text
• [15]Enyew A, Asfaw Z, Kelbessa E, Nagappan R. Status of medico-cultural commercial plants at Fiche town market, Ethiopia. Int J Pharm & H Care Res. 2013; 1(4):227-236.
• [16]Rached W, Malika MAB. Evaluation of antioxidant activity and phytochemical analysis of Osyris quadripartita. Nat Prod Chem Res. 2014; 2(5):229.
• [17]Gómez ME, Ayuso MJ, Toro MV. Activity of Osyris quadripartita salzm. methanol extract on capillary permeability in rats. Phytother Res. 1995; 9(7):528-530.
• [18]Rached W, Benamar H, Bennaceur M, Marouf A. Screening of the antioxidant potential of some Algerian indigenous plants. J Biol Sci. 2010; 10(4):316-324.
• [19]Taddese S, Asres K, Gbre-Mariam T. In vivo antimicrobial activities of some selected topically applied medical plants. Ethiop Pharma J. 2003; 21:39-41.
• [20]Geyid A, Abebe D, Debella A, Makonnen Z, Aberra F, Teka F et al.. Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles. J Ethnopharmacol. 2005; 97:421-427.
• [21]Al-Jaber HI, Mosleh IM, Mallouh A, Abu Salim OM, Abu Zarga MH. Chemical constituents of Osyris alba and their antiparasitic activities. J Asian Nat Prod Res. 2010; 12(9):814-820.
• [22]Woldemichael GM, Wink M. Concomitant occurrence of pyrrolizidine and quinolizidine alkaloids in hemiparasite Osyris alba L. (Santalaceae). Biochem Syst Ecol. 2002; 30:139-149.
• [23]Demirici F, Baser KHC. The volatiles of fresh-cut Osyris alba L. flowers. Flavour Fragr J. 2004; 19:72-73.
• [24]Kreipl AT, Konig WA. Sesquiterpenes from the east African sandalwood Osyris tenuifolia. Phytochemistry. 2004; 65:2045-2049.
• [25]Yeboah EMO, Majinda RRT, Kadziola A, Muller A. Dihydroxy-beta-agarofuran Sesquiterpenes and Pentacyclic Triterpenoids from the Root Bark of Osyris lanceolata. J Nat Prod. 2010; 73:1151-1155.
• [26]Shyaula SL, Manandhar MD, Choudhary MI. Lignans from the Nepalese sandal wood Osyris wightiana Wall ex Wight. J Nepal Chem Soc. 2011; 28:24-8.
• [27]Hedberg I, Edwards S. The flora of Ethiopia: Pittosporaceae to Araliacae. Flora of Ethiopia. Hedberg I, Edwards S, editors. Addis Ababa: National Herbarium, Biology Department, Science Faculty, Addis Ababa University, Ethopia; 1989. [vol. 3]
• [28]Herrera CM. The annual cycle of Osyris quadripartita, a hemiparasitic dioecious shrub of Mediterranean scrublands. J Ecol. 1984; 74:1065-1075.
• [29]Herrera CM. Plant size, spacing patterns, and host-plant selection in Osyris quadripartita, a hemiparasitic dioecious shrub. J Ecol. 1988; 76(4):995-1006.
• [30]USDA Germplasm Resources Information Network (GRIN) [www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl].
• [31]Enyew A, Asfaw Z, Kelbessa E, Nagappan R. Ethnobotanical study of traditional medicinal plants in and around Fiche District, central Ethiopia. Curr R J Biol Sci. 2014; 6(4):154-167.
• [32]d’Avigdor E, Wohlmuth H, Asfaw Z, Awas T. The current status of knowledge of herbal medicine and medicinal plants in Fiche. Ethiopia J Ethnobiol Ethnomed. 2014; 10:38. BioMed Central Full Text
• [33]O‘Neill MJ, Bray DH, Boardman P, Phillipson JD, Warhurst DC. Plants as source of antimalarial drugs. Part 1. In vitro test method for the evaluation of crude extracts from plants. Planta Med. 1985; 61:394-398.
• [34]Organization for Economic Co-operation and Development: OECD Guidelines for the testing of chemicals. Paris; 2008.
• [35]Organization for Economic Co-operation and Development: OECD guideline for testing of chemicals. Acute oral toxicity – Up-and-Down Procedure. Paris; 2001.
• [36]Fidock DA, Rosenthal PJ, Croft SL, Brun R, Nwaka S. Antimalarial drug discovery: efficacy models for compound screening. Nat Rev Drug Discov. 2004; 3:509-520.
• [37]Moll K, Ljungström I, Perlmann H, Scherf A, Wahlgren M. Methods in malaria research. Manassas, Virginia; 2008.
• [38]WHO: Basic malaria microscopy. Part I Learners Guide. Geneva; 2010.
• [39]Peters W, Robinson BL. The chemotherapy of rodent malarial. Studies on puronaridine and other manich base antimalarials. Annals Trop Med Parasitol. 1992; 86:455-465.
• [40]Gilmour D, Sykes AJ. Western and Wintrobe methods of estimating ESR compared. Br Med J. 1951; 2(4746):1496-1497.
• [41]Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Intern Pharma Sc. 2011; 1(1):98-106.
• [42]Cowan MM. Plant products as antimicrobial agents. Clin Microb Rev. 1999; 12:564-582.
• [43]Krettli AU, Adebayo JO, Krettli LG. Testing of natural products and synthetic molecules aiming at new antimalarials. Curr Drug Targ. 2009; 10:261-270.
• [44]Timms R, Colegrave N, Chan BHK, Read AF. The effect of parasite dose on disease severity in the rodent malaria Plasmodium chabaudi. Parasitol. 2001; 123:1-11.
• [45]Mohammed T, Erko B, Giday M. Evaluation of antimalarial activity of leaves of Acokanthera schimperi and Croton macrostachyus against Plasmodium berghei in Swiss albino mice. Compl Altern Med. 2014; 14:314. BioMed Central Full Text
• [46]Amelo W, Nagpal P, Makonnen E. Antiplasmodial activity of solvent fractions of methanolic root extract of Dodonaea angustifolia in Plasmodium berghei infected mice. Compl Altern Med. 2014; 14:462. BioMed Central Full Text
• [47]Eyasu M, Shibeshi W, Giday M. In vivo antimalarial activity of hydromethanolic extract of Calprnia auriea (Fabaceae) in mice infected with chloroquine-sensitive Plasmodium berghei. Intern J Pharm Pharmacol. 2013; 2(9):131-142.
• [48]Lamikanra AA, Brown D, Potocnik A, Casals-Pascual C, Langhorne J, Roberts DJ. Malaria anemia of mice and men. Am Soc Hematol. 2007; 110(1):18-28.
• [49]Taylor PJ, Hurd H. The influence of host haematocrit on the blood feeding success of Anopheles stephensi: implications for enhanced malaria transmission. Parasitol. 2001; 122:491-6.
• [50]Iyawe HOT, Onigbinde AO. Impact of Plasmodium berghei and chloroquine on haema antioxidant indices in mice. Asian J Biochem. 2009; 4(1):30-35.
• [51]Ejebe DE, Emudainohwo JOT, Ozako TC, Siminialayi IM, Esume CO, Maduadi UV. An investigation into the antiplasmodial effect of the ethanol extract of the leaves of Heliamthus annus in Swiss albino mice. Glob J Pharmacol. 2011; 5(2):92-96.
• [52]Tesfaye WH, Alamneh E. In vivo antimalarial activity of the crude extract and solvent fractions of the leaves of Zehenria scabra (Cucarbitacene) against Plasmodium berghei in mice. J Med Plant Res. 2014; 8(42):1230-1236.
• [53]Muzemil A. Determination of artemisinin and essential oil contents of Artimisia annua L. grown in Ethiopia and in vivo antimalarial activity of its crude extracts against Plasmodium berghei in mice. MSc thesis . Addis Ababa University, Department of Pharmaceutical Chemistry, Ethopia; 2008.