Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

BMC Microbiology

Volker F. Wendisch¹  Hironori Taniguchi¹  Ulrike Sorger-Herrmann¹ 

[1] Current adress:, Sandoz, Schaftenau, Austria

关键词: GlxR;    PhoR;    Acetate metabolism;    Carbon metabolism;    Phosphorus metabolism;    RamB;    pstS;    Phosphate starvation;    Corynebacterium glutamicum;   

Others  :  1212030
DOI  :  10.1186/s12866-015-0437-1

 received in 2014-12-18, accepted in 2015-05-05,  发布年份 2015

【 摘 要 】

Background

The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (P_i), is induced during the P_i starvation response. The two-component regulatory system PhoRS is involved in this response, but partial P_i starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR.

Results

DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many P_i starvation genes reached higher levels during the P_i starvation response on minimal medium with glucose as sole carbon source than in P_i starved acetate-grown C. glutamicum cells.

Conclusions

In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.

【 授权许可】

   
2015 Sorger-Herrmann et al.; licensee BioMed Central.

【 图 表 】

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