Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection of novel avian influenzaA (H7N9) virus

BMC Microbiology

Cheng-Feng Qin¹  Tao Jiang¹  E-De Qin¹  Qing-Yu Zhu¹  Shun-Ya Zhu¹  Yong-Qiang Deng¹  Yu Zhang¹  Xiao-Feng Li¹  Yi Hu¹  Jing Li¹  Qing-Gong Nian¹  Juan Liu² 

[1] Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongda Street, Beijing 100071, Fengtai District, China;Department of Transfusion Medicine, PLA Air Force General Hospital, Beijing 100142, China

关键词: Molecular diagnosis;    Reverse transcription-loop-mediated isothermal amplification;    H7N9 subtype;    Influenza virus;   

Others  :  1137737
DOI  :  10.1186/s12866-014-0271-x

 received in 2014-03-16, accepted in 2014-10-16,  发布年份 2014

【 摘 要 】

Background

The emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns. There is great demand for simple and rapid diagnostic method for early detection of H7N9 to provide timely treatment and disease control. The aim of the current study was to develop a rapid, accurate and feasible reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of H7N9 virus.

Results

The detection limits of the H7- and N9-specific RT-LAMP assay were both approximately 0.2 PFU per reaction. No cross-reactivity was observed with other subtype of influenza viruses or common respiratory viral pathogens. The assay worked well with clinical specimens from patients and chickens, and exhibited high specificity and sensitivity.

Conclusions

The H7/N9 specific RT-LAMP assay was sensitive and accurate, which could be a useful alternative in clinical diagnostics of influenza A (H7N9) virus, especially in the hospitals and laboratories without sophisticated diagnostic systems.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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