Hyaluronic acid increases tendon derived cell viability and collagen type I expression in vitro: Comparative study of four different Hyaluronic acid preparations by molecular weight

BMC Musculoskeletal Disorders

Anna C. Berardi²  Francesco Oliva¹  Graziella Di Bernardo³  Viviana di Giacomo⁵  Martina Berardocco²  Leonardo Osti⁴ 

[1] Department of Orthopedics and Traumatology, University of Rome “Tor Vergata” School of Medicine, Rome, Italy;U.O.C. of Immunohaematology and Transfusion Medicine, Laboratory of Stem Cells, Spirito Santo Hospital, via Fonte Romana 8, Pescara, 65125, Italy;U.O.C. of Immunohaematology and Transfusion Medicine, Santo Spirito Hospital, Pescara, Italy;Unit of Arthoscopy and Sports Trauma Surgery, Hesperia Hospital, Modena, Italy;Department of Pharmacy, University G. d’Annunzio, Chieti, Italy

关键词: Shoulder;    Rotator cuff tendons;    Human tendon derived cells;    Tendinopathy;    Hyaluronic acid;   

Others  :  1230318
DOI  :  10.1186/s12891-015-0735-7

 received in 2015-06-10, accepted in 2015-09-24,  发布年份 2015

【 摘 要 】

Background

Hyaluronic Acid (HA) has been already approved by Food and Drug Administration (FDA) for osteoarthritis (OA), while its use in the treatment of tendinopathy is still debated. The aim of this study was to evaluate in human rotator cuff tendon derived cells the effects of four different HA on cell viability, proliferation, apoptosis and the expression of collagen type I and collagen type III.

Methods

An in vitro model was developed on human tendon derived cells from rotator cuff tears to study the effects of four different HA preparations (Ps) (sodium hyaluronate MW: 500-730 KDa - Hyalgan®, 1000 kDa Artrosulfur HA®, 1600 KDa Hyalubrix® and 2200 KDa Synolis-VA®) at various concentrations. Tendon derived cells morphology were evaluated after 0, 7 and 14 d of culture. Viability, proliferation, apoptosis were evaluated after 0, 24 and 48 h of culture. The expression and deposition of collagen type I and collagen type III were evaluated after 1, 7 and 14 d of culture.

Results

All HAPs tested increased viability and proliferation, in dose dependent manner. HAPs already reduce apoptosis at 24 h compared to control cells (without HAPs). Furthermore, HAPs stimulated the synthesis of collagen type I in a dose dependent fashion over 14 d, without increase in collagen type III; moreover, in the presence of Synolis-VA® the expression and deposition of collagen type I was significantly higher as compare with the other HAPs.

Conclusions

HAPs enhanced viability, proliferation and expression of collagen type I in tendon derived cells.

【 授权许可】

   
2015 Osti et al.

【 图 表 】

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