Chemical characterization and assessment of antioxidant potentiality of Streptocaulon sylvestre Wight, an endangered plant of sub-Himalayan plains of West Bengal and Sikkim

BMC Complementary and Alternative Medicine

Tapas Kumar Chaudhuri¹  Mousumi Poddar Sarkar²  Sandipan Ray²  Priyankar Dey¹ 

[1]Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri 734013, West Bengal, India

[2]Chemical Signal and Lipidomics Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India

关键词: Streptocaulon;    Phenolic;    Lipid peroxidation;    Iron chelation;    HPLC;    Haemolytic;    GC-MS;    Free radical;    Flavonoid;    Antioxidant;   

Others  :  1172056
DOI  :  10.1186/s12906-015-0629-0

 received in 2014-11-26, accepted in 2015-03-23,  发布年份 2015

【 摘 要 】

Background

S. sylvestre Wright is an extremely rare plant, found only in the sub-Himalayan Terai region of West Bengal and neighboring Sikkim foot-hills. The plant has never been evaluated for any pharmaceutical properties. The phytochemical status of the plant is still unknown. Therefore, the aim of the study was to explore the antioxidant and free radical scavenging activities and analysis of bioactive compounds present in S. sylvestre.

Methods

S. sylvestre methanolic extract (SSME) was evaluated for different free radical scavenging activities such as hydroxyl radical, nitric oxide, singlet oxygen, hypochlorous acid, peroxynitrite, superoxide radical and hydrogen peroxide scavenging etc. Iron chelating capacity and inhibition of lipid peroxidation were studied in addition to the assessment of haemolytic activity and erythrocyte membrane stabilizing activity (EMSA). Chemical characterization of SSME were performed by high performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS).

Results

The results indicate that SSME possess potent antioxidant activity with IC₅₀ value of 113.06 ± 5.67 μg/ml, 63.93 ± 4.16 μg/ml and 142.14 ± 6.13 μg/ml for hydroxyl radical, superoxide radical and hypochlorous acid, respectively. HPLC analysis revealed presence of different phenolic secondary metabolites such as gallic acid, ferulic acid, p-coumaric acid, syringic acid, myricetin, quercetin etc. GC-MS analysis displayed the predominance of γ-sitosterol, vitamin E and squalene in SSME.

Conclusion

The present study provides a convincing evidence that S. sylvestre not only possess potent antioxidant activity but also can be used as a source of natural bioactive phytochemicals in the future.

【 授权许可】

   
2015 Dey et al.; licensee BioMed Central.

【 图 表 】

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