‘All in a box’ a concept for optimizing microbiological diagnostic sampling in prosthetic joint infections

BMC Research Notes

Trine Rolighed Thomsen¹  Henrik C Schønheyder³  Christian Pedersen²  Ole Simonsen²  Yijuan Xu⁴  Lone Heimann Larsen⁴ 

[1] Section for Medical Biotechnology, Life Science Division, Danish Technology Institute, Aarhus, Denmark;Department of Orthopaedic Surgery, Aalborg University Hospital, Aalborg, Denmark;Department of Clinical Medicine, Aalborg University, Aalborg, Denmark;Center for Microbial Communities, Institute for Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark

关键词: Transport media;    Specimen types;    Specimen handling;    Infections;    Prosthesis;   

Others  :  1132348
DOI  :  10.1186/1756-0500-7-418

 received in 2013-10-07, accepted in 2014-06-26,  发布年份 2014

【 摘 要 】

Background

Accurate microbial diagnosis is crucial for effective management of prosthetic joint infections. Culturing of multiple intraoperative tissue samples has increased diagnostic accuracy, but new preparatory techniques and molecular methods hold promise of further improvement. The increased complexity of sampling is, however, a tough challenge for surgeons and assistants in the operation theatre, and therefore we devised and tested a new concept of pre-packed boxes with a complete assortment of swabs, vials and additional tools needed in the operating theatre for non-standard samples during a clinical study of prosthetic joint infections.

Findings

The protocol for the clinical study required triplicate samples of joint fluid, periprosthetic tissue, bone tissue, and swabs from the surface of the prosthesis. Separate boxes were prepared for percutaneous joint puncture and surgical revision; the latter included containers for prosthetic components or the entire prosthesis. During a 2-year project period 164 boxes were used by the surgeons, 98 of which contained a complete set of samples. In all, 1508 (89%) of 1685 scheduled samples were received.

Conclusion

With this concept a high level of completeness of sample sets was achieved and thus secured a valid basis for evaluation of new diagnostics. Although enthusiasm for the project may have been a contributing factor, the extended project period suggests that the ‘All in a box’ concept is equally applicable in routine clinical settings with standardized but complex diagnostic sampling.

【 授权许可】

   
2014 Larsen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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