Rapid purification of gram quantities of β-sitosterol from a commercial phytosterol mixture

BMC Research Notes

Bernd Markus Lange¹  Deanna Brooke Heidorn¹  Narayanan Srividya¹ 

[1] Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA 99164, USA

关键词: Zeolite;    Phytosterol;    Chromatography;    Fractional crystallization;    Purification;    β-Sitosterol;   

Others  :  1134164
DOI  :  10.1186/1756-0500-7-182

 received in 2013-11-13, accepted in 2014-03-24,  发布年份 2014

【 摘 要 】

Background

β-Sitosterol, a plant sterol or phytosterol, has commercial uses in the nutraceutical and pharmaceutical industries, but is also employed frequently in biological research. Phytosterols always accumulate as mixtures, and obtaining highly pure β-sitosterol in larger quantities for biological assays has been a challenge.

Findings

An improved method for the rapid purification of β-sitosterol from a commercial phytosterol extract is presented. Fractional crystallization of soybean oil yielded a soluble and an insoluble fraction. β-Sitosterol was purified by silica gel and Na-Y zeolite chromatography.

Conclusion

The rapid and cost effective three-step purification described here afforded β-sitosterol in gram quantities with high purity (>92%) and yield (>22%).

【 授权许可】

   
2014 Srividya et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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