Expression pattern of Protein Kinase C ϵ during mouse embryogenesis

BMC Developmental Biology

Michael Leitges¹  Ursula Braun¹  Sergio Carracedo¹ 

[1] Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo, N-0349, Norway

关键词: Ganglia;    Lac Z;    Mouse embryogenesis;    PKC epsilon;    Novel protein kinase C;   

Others  :  1085841
DOI  :  10.1186/1471-213X-13-16

 received in 2013-01-21, accepted in 2013-04-24,  发布年份 2013

【 摘 要 】

Background

Protein kinase C epsilon (PKCϵ) belongs to the novel PKC subfamily, which consists of diacylglycerol dependent- and calcium independent-PKCs. Previous studies have shown that PKCϵ is important in different contexts, such as wound healing or cancer. In this study, we contribute to expand the knowledge on PKCϵ by reporting its expression pattern during murine midgestation using the LacZ reporter gene and immunostaining procedures.

Results

Sites showing highest PKCϵ expression were heart at ealier stages, and ganglia in older embryos. Other stained domains included somites, bone, stomach, kidney, and blood vessels.

Conclusions

The seemingly strong expression of PKCϵ in heart and ganglia shown in this study suggests a important role of this isoform in the vascular and nervous systems during mouse development. However, functional redundancy with other PKCs during midgestation within these domains and others reported here possibly exists since PKCϵ deficient mice do not display obvious embryonic developmental defects.

【 授权许可】

   
2013 Carracedo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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