Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution

BMC Microbiology

Rosa Hermosa²  Enrique Monte²  Enrique Quesada-Moraga³  Enrique A Iturriaga¹  Cándido Santiago-Álvarez³  Almudena Ortiz-Urquiza³  Marcela Márquez²  Inmaculada Garrido-Jurado² 

[1] Area de Genética. Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 324, Plaza Doctores de la Reina s/n, 37007 Salamanca, Spain;Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Departamento de Microbiología y Genética, Universidad de Salamanca, Río Duero 12, Campus de Villamayor, 37185 Salamanca, Spain;Departamento de Ciencias y Recursos Agrícolas y Forestales, Universidad de Córdoba, Edificio C4 Celestino Mutis, Campus Rabanales, 14071 Córdoba, Spain

关键词: Thermal growth;    Translation elongation factor gene;    LSU rDNA group I introns;    Entomopathogenic fungi;   

Others  :  1225232
DOI  :  10.1186/1471-2180-11-84

 received in 2010-10-26, accepted in 2011-04-26,  发布年份 2011

【 摘 要 】

Background

The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana.

Results

Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.

Conclusion

Most Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.

【 授权许可】

   
2011 Garrido-Jurado et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Wraight SP, Carruthers RI, Jaronski ST, Bradley CA, Garza CJ, Galaini-Wraight S: Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii. Biol Cont 2000, 17:203-217.
• [2]Quesada-Moraga E, Maranhao EAA, Valverde-García P, Santiago-Álvarez C: Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements and toxicogenic activity. Biol Cont 2006, 36:274-287.
• [3]Wraight SP, Jackson MA, de Kock SL: Production, stabilization and formulation of fungal biocontrol agents. In Fungi as Biocontrol Agents Progress, Problems and Potential. Edited by Butt TM, Jackson C, Magan N. Wallingford, UK: CAB International; 2001:253-287.
• [4]Enkerli J, Widmer F: Molecular ecology of fungal entomopathogens: molecular genetic tools and their applications in population and fate studies. Biocontrol 2010, 55:17-37.
• [5]Meyling NV, Eilenberg J: Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agrosystems: potential for conservation biological control. Biol Cont 2007, 43:145-155.
• [6]Kouvelis VN, Ghikas DV, Edgington S, Typas MA, Moore D: Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps). Lett Appl Microbiol 2008, 46:414-420.
• [7]Meyling NV, Lübeck M, Buckley EP, Eilenberg J, Rehner SA: Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Mol Ecol 2009, 18:1282-1293.
• [8]Rehner SA, Buckley E: A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005, 97:84-98.
• [9]Gaitan A, Valderrama AM, Saldarriaga G, Vélez P, Bustillo A: Genetic variability of Beauveria bassiana associated with the coffee berry borer Hypothenemus hampei and other insects. Mycol Res 2002, 106:1307-1314.
• [10]Aquino de Muro M, Elliott S, Moore D, Parker BL, Reid W, Bouhssini M: Molecular characterisation of Beauveria bassiana isolates obtained from overwintering sites of sunn pests (Eurygaste and Aelia species). Mycol Res 2005, 109:294-306.
• [11]Devi KU, Reineke A, Reddy NNR, Rao CUM, Padmavathi J: Genetic diversity, reproductive biology, and speciation in the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin. Genome 2006, 49:495-504.
• [12]Rehner SA, Posada F, Buckley EP, Infante F, Castillo A, Vega FE: Phylogenetic origins of African and Neotropical Beauveria bassiana s.l.. pathogens of the coffee berry borer, Hypothenemus hampei. J Invertebr Pathol 2006, 93:11-21.
• [13]Coates BS, Hellmich RL, Lewis LC: A minisatellite from the filamentous ascomycete Beauveria bassiana shows allelic variability independent of host range and geographic origin. Genome 2002, 45:125-132.
• [14]Castrillo LA, Vanderberg JD, Wraight SP: Strain-specific detection of introduced Beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers. J Invertebr Pathol 2003, 82:75-83.
• [15]Meyling NV, Eilenberg J: Occurrence and distribution of soil borne entomopathogenic fungi within a single organic agroecosystem. Agric Ecosyst Environ 2006, 113:336-341.
• [16]Aquino de Muro M, Mehta S, Moore D: The use of amplified fragment length polymorphism for molecular analysis of Beauveria bassiana isolates from Kenya and other countries, and their correlation with host and geographical origin. FEMS Microbiol Lett 2003, 229:249-257.
• [17]St Leger RJ, Allee LL, May R, Staples RC, Roberts DW: World-wide distribution of genetic variation among isolates Beauveria spp. Mycol Res 1992, 96:1007-1015.
• [18]Fernandes EKK, Moraes AML, Pacheco RS, Rangel DEN, Miller MP, Bittencourt VREP, Roberts DW: Genetic diversity among Bazilian isolates of Beauveria bassiana: comparisons with non-Brazilian isolates and other Beauveria species. J Appl Microbiol 2009, 107:760-774.
• [19]Berreta MF, Lecuona RE, Zandomeni RO, Grau O: Genotyping isolates of the entomopathogenic fungus Beauveria bassiana by RAPD with fluorescent labels. J Inverteb Pathol 1998, 71:145-150.
• [20]Bidochka MJ, Menzies FV, Kamp AM: Genetic groups of the insect-pathogenic fungus Beauveria bassiana are associated with habitat and thermal growth preferences. Arch Microbiol 2002, 178:531-537.
• [21]Ghikas DV, Kouvelis VN, Typas MA: Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii. BMC Microbiol 2010, 10:174. BioMed Central Full Text
• [22]Neuvéglise C, Brygoo Y: Identification of group-I introns in the 28S rDNA of the entomopathogenic fungus Beauveria brongniartii. Curr Genet 1994, 27:38-45.
• [23]Neuvéglise C, Brygoo Y, Riba G: 28S rDNA group I introns: a powerful tool for identifying strains of Beauveria brongniartii. Mol Ecol 1997, 6:373-381.
• [24]Coates BS, Hellmich RL, Lewis LC: Nuclear small subunit rRNA group I intron variation among Beauveria spp. provide tools for strain identification and evidence of horizontal transfer. Curr Genet 2002, 41:414-424.
• [25]Wang CS, Li Z, Typas MA, Butt TM: Nuclear large subunit rDNA group I intron distribution in a population of Beauveria bassiana strains: phylogenetic implications. Mycol Res 2003, 107:1189-1200.
• [26]Nikoh N, Fukatsu T: Evolutionary dynamics of multiple group I introns in nuclear ribosomal RNA genes of endoparasitic fungi of the genus Cordyceps. Mol Biol Evol 2001, 18:1631-1642.
• [27]Pantou M, Mavridou A, Typas MA: IGS sequence variation, group-I introns and the complete nuclear ribosomal DNA of the entomopathogenic fungus Metarhizium: excellent tools for isolate detection and phylogenetic analysis. Fungal Genet Biol 2003, 38:159-174.
• [28]Michel F, Westhof E: Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol 1990, 216:585-610.
• [29]Cech RT: Conserved sequences and structures of group I introns: building an active site for RNA catalysis. Gene 1988, 73:259-271.
• [30]Mavridou A, Cannone J, Typas MA: Identification of group I introns at three different positions within the 28S rDNA gene of the entomopathogenic fungus Metarhizium anisopliae var.anisopliae. Fungal Genet Biol 2000, 31:79-90.
• [31]Márquez M, Iturriaga EA, Quesada-Moraga E, Santiago-Álvarez C, Monte E, Hermosa R: Detection of potentially valuable polymorphisms in four group I intron insertion sites at the 3' -end of the LSU rDNA genes in biocontrol isolates of Metarhizium anisopliae. BMC Microbiol 2006, 6:77. BioMed Central Full Text
• [32]Möller EM, Bahnweg G, Sandermann H, Geiger HH: A simple and efficient protocol for the isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 1992, 20:6115-6116.
• [33]O'Donnell K, Cigelnik E, Nirenberg HI: Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 1998, 90:465-493.
• [34]Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press; 1989.
• [35]Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The Clustal × windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997, 24:4876-4882.
• [36]Felsenstein J: Confidence limits on the bootstrap: an approach using the bootstrap. Evolution 1985, 39:783-791.