Molecular characterization of hepatitis B virus in liver disease patients and asymptomatic carriers of the virus in Sudan

BMC Infectious Diseases

Anna Kramvis³  Dieter Glebe¹  Sahar Bakhiet²  Hatim Mudawi⁴  Mukhlid Yousif³ 

[1] Institute of Medical Virology, National Reference Centre of Hepatitis B and D, Justus Liebig-University of Giessen, Giessen, Germany;Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan;Hepatitis Virus Diversity Research Programme, Department of Internal Medicine, Faculty of Health Sciences, University of Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa;Department of Medicine, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

关键词: Phylogenetics;    Africa;    Subgenotype;    Sudan;    Serotype;    Genotype;    Bioinformatics;   

Others  :  1146931
DOI  :  10.1186/1471-2334-13-328

 received in 2013-02-05, accepted in 2013-07-16,  发布年份 2013

【 摘 要 】

Background

Hepatitis B virus is hyperendemic in Sudan. Our aim was to molecularly characterize hepatitis B virus from Sudanese individuals, with and without liver disease, because genotypes play an important role in clinical manifestation and treatment management.

Methods

Ninety-nine patients - 30 asymptomatic, 42 cirrhotic, 15 with hepatocellular carcinoma, 7 with acute hepatitis and 5 with chronic hepatitis- were enrolled. Sequencing of surface and basic core promoter/precore regions and complete genome were performed.

Results

The mean ± standard deviation, age was 45.7±14.8 years and the male to female ratio 77:22. The median (interquartile range) of hepatitis B virus DNA and alanine aminotransferase levels were 2.8 (2.2-4.2) log IU/ml and 30 (19–49) IU/L, respectively. Using three genotyping methods, 81/99 (82%) could be genotyped. Forty eight percent of the 99 patients were infected with genotype D and 24% with genotype E, 2% with putative D/E recombinants and 7% with genotype A. Patients infected with genotype E had higher frequency of hepatitis B e antigen-positivity and higher viral loads compared to patients infected with genotype D. Basic core promoter/precore region mutations, including the G1896A in 37% of HBeAg-negative individuals, could account for hepatitis B e antigen-negativity. Pre-S deletion mutants were found in genotypes D and E. Three isolates had the vaccine escape mutant sM133T.

Conclusion

Sudanese hepatitis B virus carriers were mainly infected with genotypes D or E, with patients infected with genotype E having higher HBeAg-positivity and higher viral loads. This is the first study to molecularly characterize hepatitis B virus from liver disease patients in Sudan.

【 授权许可】

   
2013 Yousif et al.; licensee BioMed Central Ltd.

【 图 表 】

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