Mycelial mass production of fungi Duddingtonia flagrans and Monacrosporium thaumasium under different culture conditions

BMC Research Notes

Marcos Pezzi Guimarães³  Walter dos Santos Lima³  Filippe Elias F Soares⁵  Luana Alcântara Borges²  Fabio Ribeiro Braga⁴  Jackson Victor de Araújo²  Manoel Eduardo da Silva¹ 

[1] URECO/EPAMIG, Pitangui, MG, Brasil;Departamento de Veterinária, UFV, Viçosa, MG, Brasil;Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brasil;Universidade Vila Velha- UVV, Vila Velha, ES, Brasil;Departamento de Bioquímica e Biologia Molecular, UFV, Viçosa, MG, Brasil

关键词: Fungal mycelium;    Biological control;    Monacrosporium thaumasium;    Duddingtonia flagrans;    Nematophagous fungi;   

Others  :  1141800
DOI  :  10.1186/1756-0500-6-340

 received in 2013-04-04, accepted in 2013-08-21,  发布年份 2013

【 摘 要 】

Background

Duddingtonia flagrans and Monacrosporium thaumasium are promising fungus species in veterinary biological control of gastrointestinal nematodes because of their production capacity of fungal structures (conidia and/or chlamydospores), growth efficiency in laboratory solid media and especially their predatory capacity. However, their large-scale production remains a challenge. This work aimed at evaluating the mycelial mass production of D. flagrans (AC001 and CG722) and M. thaumasium (NF34A) nematophagous fungi under different culture conditions.

Results

The results did not present significant differences (p > 0.05) in mycelia mass production between the isolates cultured under pH 4.0. Furthermore, after 168 hrs., the isolate CG722 presented a lower production of mycelial mass in medium CM (corn meal) (p < 0.05).

Conclusion

We therefore concluded the use of culture media SD (soy dextrose) and CG (corn grits) at pH values between 6.0 and 7.0 is suitable for high mycelial mass production of D. flagrans and M. thaumasium.

【 授权许可】

   
2013 da Silva et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Araújo JV, Mota MA, Campos AK: Controle biológico de helmintos parasitos de animais por fungos nematófagos. Rev Bras Parasitol Vet 2004, 13(1):165-169.
• [2]Braga FR, Araújo JV, Araújo JM, Tavela AO, Ferreira SR, Soares FEF, Benjamin LA, Frassy LN: Influence of the preservation period in silica-gel on the predatory activity of the isolates of Duddingtonia flagrans on infective larvae of cyathostomins (Nematoda: Cyathostominae). Exp Parasitol 2011, 128:460-463.
• [3]Carvalho RO, Braga FR, Araújo JV: Viability and nematophagous activity of the freeze-dried fungus Arthrobotrys robusta against Ancylostoma spp. infective larvae in dogs. Vet Parasitol 2011, 176:236-239.
• [4]Mota MA, Campos AK, Araújo JV: Influence of different storage methods on the predatory capacity of the fungi Arthrobotrys robusta and Monacrosporium thaumasium after passage through the bovine gastrointestinal tract. World J Microbiol Biotechnol 2003, 19:913-916.
• [5]Campos AK, Araújo JV, Mota MA, Cecon PR: Atividade predatória, crescimento radial e esporulação de fungos predadores de nematóides Monacrosporium spp, submetidos à criopreservação. Ciência Rural 2004, 34(2):465-469.
• [6]Nozaki MH, Camargo M, Barreto M: Caracterização de Diaporthe citri em diferentes meios de culturas, condições de temperatura e luminosidade. Fitopato Bras 2004, 29(4):429-432.
• [7]Magalhães KA, Valadares Filho SC, Pereira OG: Tabelas de composição de alimentos. In Exigências nutricionais de zebuínos e tabelas de composição de alimentos BR-corte. 1st edition. Viçosa: UFV; 2006:95-142.
• [8]Rosenweig WD: Role of aminoacids, peptides and medium composition in trap formation by nematode-trapping fungi. Can J Microbiol 1983, 30:265-267.
• [9]Dijksterhuis J, Harder W, Veenhuis M: Proliferation and function of the microbodies in the nematophagous fungus Arthrobotrys oligospora during growth on oleic acid or D-alanine as the sole carbon source. FEMS Microbiol Letters 1993, 94:1-9.
• [10]Araújo JV, Ribeiro R: Atividade predatória sobre larvas de tricostrongilídeos (nematoda: trichostrongyloidea) de isolados fúngicos do gênero Monacrosporium após a passagem pelo trato gastrintestinal de bovinos. Rev Bras Parasitol Vet 2003, 12:76-81.
• [11]Dackman C, Olsson S, Jansson HB, Lundgren B: Nordbring-Hertz B: Quantification of predatory and endoparasitic nematophagous fungi and their activies in soil. Microb Ecol 1987, 13:89-93.
• [12]Santos MA, Ferraz S, Muchovej J: Detection and ecology of nematophagous fungi from Brazil soils. Nemato Bras 1991, 15(2):121-134.
• [13]Gomes APS, Araújo JV, Ribeiro RCF: Differential in vitro pathogenicity of predatory fungi of the genus Monacrosporium for phytonematodes, free-living nematodes and parasitic nematodes of cattle. Braz J Med Biol Res 1999, 32(1):79-83.
• [14]Araújo JV, Gomes APS, Guimarães MP: Biological control of bovine gastrointestinal nematode parasites in southern Brazil by the nematode-trapping fungus Arthrobotrys robusta. Rev Bras Parasitol Vet 1998, 7:117-122.
• [15]Larsen M: Biological control of helminths. International J Parasitol 1999, 29:139-146.
• [16]Soprunov FF: Predacious hyphomycetous fungi and their application to the control of pathogenic nematodes. Ashkhabad: Academy of Science Turkkmen; 1958.
• [17]Mitsui Y: Distribution and ecology of nematode-trapping fungi in Japan. JARQ 1985, 18:182-193.
• [18]Dias WP, Ferraz S: Crescimento e esporulação de Arthrobotrys spp em diferentes substratos, meios de cultura, pH e níveis de temperatura. Nemato Bras 1993, 17(2):168-181.
• [19]Gorlenko MV, Kondakova EI, Pletneva OI: On the biology of predacious fungi. Bull Moscow Assoc Exper Biol 1959, 64:89-97.
• [20]Dhingra OD, Sinclair JB: Basic plant pathology methods. Second edition. CRC Press; 1995:434.
• [21]Menezes M, Silva-Hanlin DMW: Guia prático para fungos fitopatogênicos. Recife: UFRPE; 1997.