Intrapulmonary administration of bone-marrow derived M1/M2 macrophages to enhance the resolution of LPS-induced lung inflammation: noninvasive monitoring using free-breathing MR and CT imaging protocols

BMC Medical Imaging

Mohammed Alnafea²  Asma Sultana Shaik¹  Achraf Al Faraj² 

[1] Prince Naif Health Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia;Molecular & Cellular Imaging Lab, Department of Radiological Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia

关键词: Computer tomography;    Magnetic resonance imaging;    Noninvasive pulmonary imaging;    Macrophages polarization;    Cell labeling and tracking;    Lung inflammation;   

Others  :  1217028
DOI  :  10.1186/s12880-015-0059-y

 received in 2015-03-10, accepted in 2015-05-13,  发布年份 2015

【 摘 要 】

Background

Alveolar macrophages, with their high functional plasticity, were reported to orchestrate the induction and resolution of inflammatory processes in chronic pulmonary diseases. Noninvasive imaging modalities that offer simultaneous monitoring of inflammation progression and tracking of macrophages subpopulations involved in the inflammatory cascade, can provide an ideal and specific diagnostic tool to visualize the action mechanism in its initial stages. Therefore, the purpose of the current study was to evaluate the role of M1 and M2 macrophages in the resolution of lipopolysaccharide (LPS)-induced lung inflammation and monitor this process using noninvasive free-breathing MRI and CT protocols.

Methods

Bone-marrow derived macrophages were first polarized to M1 and M2 macrophages and then labeled with superparamagnetic iron oxide nanoparticles. BALB/c mice with lung inflammation received an intrapulmonary instillation of these ex vivo polarized M1 or M2 macrophages. The biodistribution of macrophages subpopulations and the subsequent resolution of lung inflammation were noninvasively monitored using MRI and micro-CT. Confirmatory immunohistochemistry analyses were performed on lung tissue sections using specific macrophage markers.

Results

As expected, large inflammatory areas noninvasively imaged using pulmonary MR and micro-CT were observed within the lungs following LPS challenge. Subsequent intrapulmonary administration of M1 and M2 macrophages resulted in a significant decrease in inflammation starting from 72 h. Confirmatory immunohistochemistry analyses established a progression of lung inflammation with LPS and its subsequent reduction with both macrophages subsets. An enhanced resolution of inflammation was observed with M2 macrophages compared to M1.

Conclusions

The current study demonstrated that ex vivo polarized macrophages decreased LPS-induced lung inflammation. Noninvasive free-breathing MR and CT imaging protocols enabled efficient monitoring of progression and resolution of lung inflammation.

【 授权许可】

   
2015 Al Faraj et al.; licensee BioMed Central.

【 图 表 】

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