TypA is involved in virulence, antimicrobial resistance and biofilm formation in Pseudomonas aeruginosa

BMC Microbiology

Joerg Overhage²  Olivier Lesouhaitier³  Martina Rueger²  Nikola Strempel²  Beatrix Tettmann²  Thibaut Rosay³  Amy TY Yeung¹  Anke Neidig² 

[1] Centre for Microbial Diseases & Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, BC, Canada;Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, 76021, Germany;Laboratory of Microbiology Signals and Microenvironment, LMSM EA 4312, University of Rouen, 55 rue Saint Germain, Evreux, 27000, France

关键词: Resistance;    Biofilm;    Macrophage;    Dictyostelium discoideum;    Virulence;    Type III secretion system;    TypA;    Pathogen;    Pseudomonas aeruginosa;   

Others  :  1143955
DOI  :  10.1186/1471-2180-13-77

 received in 2012-10-30, accepted in 2013-04-04,  发布年份 2013

【 摘 要 】

Background

Pseudomonas aeruginosa is an important opportunistic human pathogen and is extremely difficult to treat due to its high intrinsic and adaptive antibiotic resistance, ability to form biofilms in chronic infections and broad arsenal of virulence factors, which are finely regulated. TypA is a GTPase that has recently been identified to modulate virulence in enteric Gram-negative pathogens.

Results

Here, we demonstrate that mutation of typA in P. aeruginosa resulted in reduced virulence in phagocytic amoebae and human macrophage models of infection. In addition, the typA mutant was attenuated in rapid cell attachment to surfaces and biofilm formation, and exhibited reduced antibiotic resistance to ß-lactam, tetracycline and antimicrobial peptide antibiotics. Quantitative RT-PCR revealed the down-regulation, in a typA mutant, of important virulence-related genes such as those involved in regulation and assembly of the Type III secretion system, consistent with the observed phenotypes and role in virulence of P. aeruginosa.

Conclusions

These data suggest that TypA is a newly identified modulator of pathogenesis in P. aeruginosa and is involved in multiple virulence-related characteristics.

【 授权许可】

   
2013 Neidig et al.; licensee BioMed Central Ltd.

【 图 表 】

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