Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure

BMC Gastroenterology

Anson W Lowe¹  M Bishr Omary²  Peyman Sahbaie²  Zheng Wang³  Jong Hyeok Kim³  George Triadafilopoulos³  Sumita Sood³  Ying Hao³ 

[1]Stanford University Digestive Disease Center, Stanford, CA, USA

[2]Gastroenterology Section, Palo Alto Veterans Affairs Health Care System, Palo Alto, CA, USA

[3]Department of Medicine, Stanford University, Stanford, CA, USA

Others  :  1211647
DOI  :  10.1186/1471-230X-7-24

 received in 2007-01-19, accepted in 2007-06-27,  发布年份 2007

【 摘 要 】

Background

Esophageal reflux and Barrett's esophagus represent two major risk factors for the development of esophageal adenocarcinoma. Previous studies have shown that brief exposure of the Barrett's-associated adenocarcinoma cell line, SEG-1, or primary cultures of Barrett's esophageal tissues to acid or bile results in changes consistent with cell proliferation. In this study, we determined whether similar exposure to acid or bile salts results in gene expression changes that provide insights into malignant transformation.

Methods

Using previously published methods, Barrett's-associated esophageal adenocarcinoma cell lines and primary cultures of Barrett's esophageal tissue were exposed to short pulses of acid or bile salts followed by incubation in culture media at pH 7.4. A genome-wide assessment of gene expression was then determined for the samples using cDNA microarrays. Subsequent analysis evaluated for statistical differences in gene expression with and without treatment.

Results

The SEG-1 cell line showed changes in gene expression that was dependent on the length of exposure to pH 3.5. Further analysis using the Gene Ontology, however, showed that representation by genes associated with cell proliferation is not enhanced by acid exposure. The changes in gene expression also did not involve genes known to be differentially expressed in esophageal adenocarcinoma. Similar experiments using short-term primary cultures of Barrett's esophagus also did not result in detectable changes in gene expression with either acid or bile salt exposure.

Conclusion

Short-term exposure of esophageal adenocarcinoma SEG-1 cells or primary cultures of Barrett's esophagus does not result in gene expression changes that are consistent with enhanced cell proliferation. Thus other model systems are needed that may reflect the impact of acid and bile salt exposure on the esophagus in vivo.

【 授权许可】

   
2007 Hao et al; licensee BioMed Central Ltd.

【 图 表 】

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