Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae

BMC Evolutionary Biology

Monique Turmel¹  Christian Otis¹  Claude Lemieux¹ 

[1] Département de Biochimie, de Microbiologie et de Bio-informatique, Institut de Biologie Intégrative et des Systèmes, Université Laval, 1030 avenue de la Medicine, Pavillon Marchand, Québec, G1V 0A6, Canada

关键词: Phylogenomics;    Plastid genome;    Trebouxiophyceae;    Chlorophyta;   

Others  :  1117924
DOI  :  10.1186/s12862-014-0211-2

 received in 2014-07-24, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

The green algae represent one of the most successful groups of photosynthetic eukaryotes, but compared to their land plant relatives, surprisingly little is known about their evolutionary history. This is in great part due to the difficulty of recognizing species diversity behind morphologically similar organisms. The Trebouxiophyceae is a species-rich class of the Chlorophyta that includes symbionts (e.g. lichenized algae) as well as free-living green algae. Members of this group display remarkable ecological variation, occurring in aquatic, terrestrial and aeroterrestrial environments. Because a reliable backbone phylogeny is essential to understand the evolutionary history of the Trebouxiophyceae, we sought to identify the relationships among the major trebouxiophycean lineages that have been previously recognized in nuclear-encoded 18S rRNA phylogenies. To this end, we used a chloroplast phylogenomic approach.

Results

We determined the sequences of 29 chlorophyte chloroplast genomes and assembled amino acid and nucleotide data sets derived from 79 chloroplast genes of 61 chlorophytes, including 35 trebouxiophyceans. The amino acid- and nucleotide-based phylogenies inferred using maximum likelihood and Bayesian methods and various models of sequence evolution revealed essentially the same relationships for the trebouxiophyceans. Two major groups were identified: a strongly supported clade of 29 taxa (core trebouxiophyceans) that is sister to the Chlorophyceae + Ulvophyceae and a clade comprising the Chlorellales and Pedinophyceae that represents a basal divergence relative to the former group. The core trebouxiophyceans form a grade of strongly supported clades that include a novel lineage represented by the desert crust alga Pleurastrosarcina brevispinosa. The assemblage composed of the Oocystis and Geminella clades is the deepest divergence of the core trebouxiophyceans. Like most of the chlorellaleans, early-diverging core trebouxiophyceans are predominantly planktonic species, whereas core trebouxiophyceans occupying more derived lineages are mostly terrestrial or aeroterrestrial algae.

Conclusions

Our phylogenomic study provides a solid foundation for addressing fundamental questions related to the biology and ecology of the Trebouxiophyceae. The inferred trees reveal that this class is not monophyletic; they offer new insights not only into the internal structure of the class but also into the lifestyle of its founding members and subsequent adaptations to changing environments.

【 授权许可】

   
2014 Lemieux et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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Figure 4.

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