The nutritional composition and anti-obesity effects of an herbal mixed extract containing Allium fistulosum and Viola mandshurica in high-fat-diet-induced obese mice

BMC Complementary and Alternative Medicine

Ho Kyoung Kim¹  Byoung Wan Yoo²  Seung-Hyung Kim³  Yoon-Young Sung¹ 

[1] Mibyeong Research Center, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu 305-811, Daejeon, Republic of Korea;Institute of JinAn Red Ginseng, Jinan-Eup, Jinan-Gun 567-801, Chonbuk, Republic of Korea;Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 300-716, Republic of Korea

关键词: Vitamin;    Viola mandshurica;    Nutritional;    Mixture;    Hyperlipidemia;    Allium fistulosum;   

Others  :  1233240
DOI  :  10.1186/s12906-015-0875-1

 received in 2015-07-05, accepted in 2015-09-23,  发布年份 2015

【 摘 要 】

Background

In traditional oriental medicine, A. fistulosum and V. mandshurica are considered to be effective in promoting blood circulation. Therefore, in this study, we investigated whether a solution containing both A. fistulosum and V. mandshurica (AFE + VME) extracts has synergistic effects on the treatment of hyperlipidemia and obesity.

Methods

Anti-obesity effects of an herbal extract containing Allium fistulosum and Viola mandshurica (AFE + VME) were investigated in high-fat diet (HFD)-induced obese mice. AFE + VME was orally administrated to mice with the HFD at a dose of 200 mg/kg/day for 8 weeks. We observed the effects of mixed extract on body weight, fat mass, serum lipid levels, and mRNA expression levels of lipid metabolism-related genes in the adipose tissue of mice.

Results

The nutritional analysis revealed that this mixed extract is high in carbohydrate (72.2 g/100 g) and protein (11.5 g/100 g); low in fat (1.7 g/100 g); rich in vitamins E (4.8 mg/100 g), B ₁(14.8 mg/100 g), B ₂(1.0 mg/100 g), niacin (7.9 mg/100 g), and folic acid (1.57 mg/100 g); and rich in minerals such as calcium (600 mg/100 g), iron (106.1 mg/100 g), and zinc (5.8 mg/100 g). The oral administration of AFE + VME in obese mice reduced body weight, tissue weight, adipocyte size, and lipid accumulation in the liver compared with HFD control mice. AFE + VME also decreased serum triglyceride, total cholesterol, and leptin concentrations. Furthermore, AFE + VME markedly increased the mRNA expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), uncoupling protein-2 (UCP-2), and adiponectin and decreased leptin expression in the epididymal white adipose tissue. Our results suggest that the extract containing A. fistulosum and V. mandshurica improved lipid metabolism via the up-regulation of PPAR-γ, UCP-2, and adiponectin expression and the down-regulation of leptin in HFD-induced obese mice.

Conclusions

Therefore, the extract containing Allium fistulosum and Viola mandshurica may be a potentially effective therapy for obesity and its related metabolic disorders such as hyperlipidemia and insulin resistance.

【 授权许可】

   
2015 Sung et al.

【 图 表 】

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【 参考文献 】

• [1]Fenwick GR, Hanley AB. The genus Allium-Part 1. Crit Rev Food Sci Nutr. 1985; 22(3):199-271.
• [2]Chen JH, Chen HI, Wang JS, Tsai SJ, Jen CJ. Effects of Welsh onion extracts on human platelet function in vitro. Life Sci. 2000; 66:1571-9.
• [3]Yamamoto Y, Aoyama S, Hamaguchi N, Rhi GS. Antioxidative and antihypertensive effects of Welsh onion on rats fed with a high-fat high-sucrose diet. Biosci Biotechnol Biochem. 2005; 69:1311-7.
• [4]Yamamoto Y, Yasuoka A. Welsh onion attenuates hyperlipidemia in rats fed on high-fat high-sucrose diet. Biosci Biotechnol Biochem. 2010; 74(2):402-4.
• [5]Jeon GI, Yoon MY, Park HR, Lee SC, Park E. Neuroprotective activity of Viola mandshurica extracts on hydrogen peroxide-induced DNA damage and cell death in PC12 dells. Ann N York Acad Sc. 2009; 1171:576-82.
• [6]Lee BB, Park SR, Han CS, Han DY, Park EJ, Park HR et al.. Antioxidant activity and inhibition activity against a-amylase and a-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr. 2008; 37:405-9.
• [7]Hongyuan X. Oriental material medica: a concise guide. Oriental Healing Arts institute, Long Beach; 1986.
• [8]Lee BW, Kim DH. Inhibitory effect of Violae herba extract on inflammatory cytokine production by IL-1β and TNF-α in cultured human synovial cells. Kor J Herbol. 2003; 18:89-96.
• [9]Devlin MJ, Yanovski SZ, Wilson GT. Obesity: what mental health professionals need to know. Am J Psychiatry. 2000; 157(6):854-66.
• [10]Yun JM. Possible anti-obesity therapeutics from nature – A review. Phytochemistry. 2010; 71:1625-41.
• [11]Sung YY, Yoon Y, Kim SJ, Yang WK, Kim HK. Anti-obesity activity of Allium fistulosum L. extract by downregulation of the expression of lipogenic genes in high-fat diet-induced obese mice. Mol Med Rep. 2011; 4(3):431-5.
• [12]Sung YY, Kim DS, Kim HK. Viola mandshurica ethanolic extract prevents high-fat-diet-induced obesity in mice by activating AMP-activated protein kinase. Environ Toxicol Pharmacol. 2014; 38:41-50.
• [13]Kang JS, Lee WK, Lee CW, Yoon WK, Kim N, Park SK et al.. Improvement of high-fat diet-induced obesity by a mixture of red grape extract, soy isoflavone and L-carnitine: implications in cardiovascular and non-alcoholic fatty liver diseases. Food Chem Toxicol. 2011; 49(9):2453-8.
• [14]Shen XH, Tang QY, Huang J, Cai W. Vitamin E regulates adipocytokine expression in a rat model of dietary-induced obesity. Exp Biol Med (Maywood). 2010; 235(1):47-51.
• [15]Tanaka T, Kono T, Terasaki F, Yasui K, Soyama A, Otsuka K et al.. Thiamine prevents obesity and obesity-associated metabolic disorders in OLETF rats. J Nutr Sci Vitaminol (Tokyo). 2010; 56(6):335-46.
• [16]Sarna LK, Wu N, Wang P, Hwang SY, Siow YL OK. Folic acid supplementation attenuates high fat diet induced hepatic oxidative stress via regulation of NADPH oxidase. Can J Physiol Pharmacol. 2012; 90:155-65.
• [17]Wanders D, Graff EC, White BD, Judd RL. Niacin increases adiponectin and decreases adipose tissue inflammation in high fat diet-fed mice. PLoS One. 2013; 8(8):e71285.
• [18]Charradi K, Elkahoui S, Limam F, Aouani E. High-fat diet induced an oxidative stress in white adipose tissue and disturbed plasma transition metals in rat: prevention by grape seed and skin extract. J Physiol Sci. 2013; 63(6):445-55.
• [19]Brand MD, Esteves TC. Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3. Cell Metab. 2005; 2(2):85-93.
• [20]Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y et al.. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med. 1995; 1:1155-61.
• [21]Chandran M, Phillips SA, Ciaraldi T, Henry RR. Adiponectin: more than just another fat cell hormone? Diabetes Care. 2003; 26(8):2442-50.
• [22]Clark M, Thomaseth K, Dirikolu L, Ferguson DC, Hoenig M. Effects of pioglitazone on insulin sensitivity and serum lipids in obese cats. J Vet Intern Med. 2014; 28(1):166-74.
• [23]Matsuo K, Arai H, Muto K, Fukaya M, Sato T, Mizuno A et al.. The anti-obesity effect of the palatinose-based formula inslow is likely due to an increase in the hepatic PPAR-alpha and adipocyte PPAR-gamma gene expressions. J Clin Biochem Nutr. 2007; 40:234-41.
• [24]Maeda N, Takahashi M, Funahashi T, Kihara S, Nishizawa H, Kishida K et al.. PPAR gamma ligands increase expression and plasma concentrations of adiponectin an adipose derived protein. Diabetes. 2001; 50:2094-9.
• [25]Sung YY, Kim SH, Kim SD, Park SH, Yoo BW, Kim HK. Nutritional composition and anti-obesity effects of cereal bar containing Allium Fistulosum (welsh onion) extract. J Funct Foods. 2004; 6:428-37.
• [26]Hong JL, Zhou HY, Zhu J, Li L, Shu P, Qin XY et al.. Determination and comparative analysis of major constituents in Viola yedoensis Makino and different species from genus Viola by high-performance liquid chromatography with chemometrics methods. J Med Plants Res. 2011; 5:5230-9.
• [27]Karmase A, Birari R, Bhutani K. Evaluation of anti-obesity effect of Aegle Marmelos leaves. Phytomedicine. 2013; 20:805-12.
• [28]Son MJ, Rico CW, Nam SH, Kang MY. Influence of oryzanol and ferulic acid on the lipid metabolism and antioxidative status in high fat-fed mice. J Clin Biochem Nutr. 2010; 46:150-6.
• [29]Rivera L, Moron M, Sanchez M, Zarzuelo A, Galisteo M. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese zucker rats. Obesity. 2008; 16:2081-7.