Shorter daily dwelling time in peritoneal dialysis attenuates the epithelial-to-mesenchymal transition of mesothelial cells

BMC Nephrology

Yuan-Yow Chiou⁷  Hao-Kuang Wang¹  Yung-Tang Kung⁴  Kuang-Wen Liu⁴  Hong-Arh Fan⁴  Hsin-Pao Chen⁴  Yi-Ting Chen³  Li-Chun Ho⁵  Hsi-Hao Wang³  Min-Yu Chang³  Hsiang-Chun Liu⁶  Hung-Hsiang Liou²  Sheng-Hsiang Lin⁵  Tsun-Mei Lin⁸  Shih-Yuan Hung⁹  Yau-Sheng Tsai⁵  Yi-Che Lee⁵ 

[1] Department of Neurosurgery, E-DA Hospital/I-Shou University, Kaohsiung, Taiwan;Division of Nephrology, Department of Medicine, Hsin-Jen Hospital, New Taipei City, Taiwan;Division of Nephrology, Department of Internal Medicine, E-DA Hospital/I-Shou University, Kaohsiung, Taiwan;Division of Colorectal Surgery, Department of Surgery, E-DA Hospital/I-Shou University, Kaohsiung, Taiwan;Institute of Clinical Medicine, National Cheng Kung University, Tainan, Taiwan;Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan;Department of Pediatrics, National Cheng Kung University Hospital, Medical College, No. 138, Sheng-Li Road, Tainan City 701, Taiwan;Department of Laboratory Medicine, E-DA Hospital/I-Shou University, Kaohsiung, Taiwan;Department of Health Management, I-Shou University, Kaohsiung, Taiwan

关键词: Peritoneal dialysis;    Mesothelial cells;    Epithelial-to-mesenchymal transition;    Dwelling time;   

Others  :  1082724
DOI  :  10.1186/1471-2369-15-35

 received in 2013-02-05, accepted in 2014-02-14,  发布年份 2014

【 摘 要 】

Background

Peritoneal dialysis (PD) therapy is known to induce morphological and functional changes in the peritoneal membrane. Long-term exposure to conventional bio-incompatible dialysate and peritonitis is the main etiology of inflammation. Consequently, the peritoneal membrane undergoes structural changes, including angiogenesis, fibrosis, and hyalinizing vasculopathy, which ultimately results in technique failure. The epithelial-to-mesenchymal transition (EMT) of mesothelial cells (MCs) plays an important role during the above process; however, the clinical parameters associated with the EMT process of MCs remain to be explored.

Methods

To investigate the parameters impacting EMT during PD therapy, 53 clinical stable PD patients were enrolled. EMT assessments were conducted through human peritoneal MCs cultured from dialysate effluent with one consistent standard criterion (MC morphology and the expression of an epithelial marker, cytokeratin 18). The factors potentially associated with EMT were analyzed using logistic regression analysis. Primary MCs derived from the omentum were isolated for the in vitro study.

Results

Forty-seven percent of the patients presented with EMT, 28% with non-EMT, and 15% with a mixed presentation. Logistic regression analysis showed that patients who received persistent PD therapy (dwelling time of 24 h/day) had significantly higher EMT tendency. These results were consistent in vitro.

Conclusions

Dwelling time had a significant effect on the occurrence of EMT on MCs.

【 授权许可】

   
2014 Lee et al.; licensee BioMed Central Ltd.

【 图 表 】

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