Rapid research response to the 2009 A(H1N1)pdm09 influenza pandemic (Revised)

BMC Research Notes

Flor Munoz⁴  Susan Fisher-Hoch¹  Rosanna Lagos⁶  Rebecca A Halpin³  Jill Barrett⁷  Hana M El Sahly²  Gail Demmler⁴  Robert L Atmar²  Pedro A Piedra⁴  Wendy A Keitel⁵ 

[1] University of Texas School of Public Health, Brownsville, TX, USA;Department Medicine, Houston, TX, USA;J. Craig Venter Institute, Rockville, MD, USA;Department Pediatrics, Baylor College of Medicine, Houston, TX, USA;Departments of Molecular Virology & Microbiology and Medicine, Baylor College of Medicine, Room 280, One Baylor Plaza, Houston, TX 77030, USA;Centro para Vacunas en Desarrollo-Chile, Santiago, Chile;The EMMES Corporation, Rockville, MD, USA

关键词: Influenza;    Immune responses;    2009 H1N1 virus;   

Others  :  1142769
DOI  :  10.1186/1756-0500-6-177

 received in 2012-08-01, accepted in 2013-03-23,  发布年份 2013

【 摘 要 】

Background

When novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines. The objective of the study was to collect samples from patients with suspected or confirmed A(H1N1)pdm09 infections that could be made available to the scientific community. Respiratory secretions, sera and peripheral blood mononuclear cells (PBMCs) were collected sequentially (when possible) from patients presenting with suspected or previously confirmed A(H1N1)pdm09 infections. Clinical manifestations and illness outcomes were assessed. Respiratory secretions were tested for the presence of A(H1N1)pdm09 virus by means of isolation in tissue culture and real time RT-PCR. Sera were tested for the presence and level of HAI and neutralizing antibodies against the A(H1N1)pdm09 virus.

Findings and conclusions

Thirty patients with confirmed A(H1N1)pdm09 infection were enrolled at Baylor College of Medicine (BCM). Clinical manifestations of illness were consistent with typical influenza. Twenty-eight of 30 had virological confirmation of illness; all recovered fully. Most patients had serum antibody responses or high levels of antibody in convalescent samples. Virus-positive samples were sent to J. Craig Venter Institute for sequencing and sequences were deposited in GenBank. Large volumes of sera collected from 2 convalescent adults were used to standardize antibody assays; aliquots of these sera are available from the repository. Aliquots of serum, PBMCs and stool collected from BCM subjects and subjects enrolled at other study sites are available for use by the scientific community, upon request.

【 授权许可】

   
2013 Keitel et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Neumann G, Noda T, Kawaoka Y: Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 2009, 459:931-939.
• [2]Centers for Disease Control and Prevention: Swine influenza A (H1N1) infection in two children-Southern California, March-April 2009. MMWR Morbid Mortal Wkly Rep 2009, 58:400-402.
• [3]World Health Organization: World now at the start of 2009 influenza pandemic. http://who.int/mediacentre/news/statements/2009/h1n1_pandemic_phase6_20090611/en/index.html webcite
• [4]Centers for Disease Control and Prevention: CDC protocol of real time RTPCR for influenza A (H1N1). April 2009. http://www.who.int/csr/resources/publications/swineflu/realtimeptpcr/en/index.html webcite
• [5]Jackson LA, Patel SM, Swamy GK: Immunogenicity of an inactivated monovalent 2009 H1N1 influenza vaccine in pregnant women. J Infect Dis 2011, 204:854-863.
• [6]Papenburg J, Baz M, Hamelin M-E: Household transmission of the 2009 pandemic A/H1N1 influenza virus: elevated laoratory-confirmed secondary attack rates and evidence of asymptomatic infections. Clin Infect Dis 2010, 51:1033-1041.
• [7]Cauchemez S, Donnellly CA, Reed C: Household transmission of 2009 pandemic influenza a (H1N1) virus in the United States. N Engl J Med 2009, 361:2619-2627.
• [8]Faix DJ, Sherman SS, Waterman SH: Rapid-test sensitivity for novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 2009, 361:728-729.
• [9]Centers for Disease Control and Prevention: Oseltamivir-resistant 2009 pandemic influenza A (H1N1) virus infection in two summer campers receiving prophylaxis-North Carolina, 2009. MMWR Morbid Mortality Wkly Rep 2009, 58:969-972.
• [10]Gaines WJ, Ballou J, Yan C: Utilizing spatiotemporal analysis of influenza-like illness and rapid tests to focus swine-origin influenza virus intervention. Health Place 2010, 16:1230-1239.
• [11]McCormick JB, Yan C, Ballou J: Response to H1N1 in a U.S.-Mexico border community. Biosecur Bioterror 2010, 8:233-242.