The VieB auxiliary protein negatively regulates the VieSA signal transduction system in Vibrio cholerae

BMC Microbiology

Andrew Camilli¹  Sophia A Kenrick²  Ayman M Ismail¹  Stephanie L Mitchell¹ 

[1] Howard Hughes Medical Institute and the Department of Molecular Biology and Microbiology, Tufts University, School of Medicine, Boston, USA;Wyatt Technology Corporation, Santa Barbara, USA

关键词: Vibrio cholerae;    Tetratricopeptide repeat domain;    Phosphodiesterases;    Protein phosphorylation;    Bacterial signal transduction;    Phosphorelay;    Two-component system;   

Others  :  1135395
DOI  :  10.1186/s12866-015-0387-7

 received in 2014-12-03, accepted in 2015-02-13,  发布年份 2015

【 摘 要 】

Background

Vibrio cholerae is a facultative pathogen that lives in the aquatic environment and the human host. The ability of V. cholerae to monitor environmental changes as it transitions between these diverse environments is vital to its pathogenic lifestyle. One way V. cholerae senses changing external stimuli is through the three-component signal transduction system, VieSAB, which is encoded by the vieSAB operon. The VieSAB system plays a role in the inverse regulation of biofilm and virulence genes by controlling the concentration of the secondary messenger, cyclic-di-GMP. While the sensor kinase, VieS, and the response regulator, VieA, behave similar to typical two-component phosphorelay systems, the role of the auxiliary protein, VieB, is unclear.

Results

Here we show that VieB binds to VieS and inhibits its autophosphorylation and phosphotransfer activity thus preventing phosphorylation of VieA. Additionally, we show that phosphorylation of the highly conserved Asp residue in the receiver domain of VieB regulates the inhibitory activity of VieB.

Conclusion

Taken together, these data point to an inhibitory role of VieB on the VieSA phosphorelay, allowing for additional control over the signal output. Insight into the function and regulatory mechanism of the VieSAB system improves our understanding of how V. cholerae controls gene expression as it transitions between the aquatic environment and human host.

【 授权许可】

   
2015 Mitchell et al.; licensee BioMed Central.

【 图 表 】

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