Prevalence and antimicrobial susceptibility profile of listeria species from ready-to-eat foods of animal origin in Gondar Town, Ethiopia

BMC Microbiology

Tamiru Birhanu³  Abraham Fikru⁴  Mebrat Ejo¹  Elias Kebede¹  Seleshe Nigatu¹  Tigist Biru¹  Ayele Taddese⁵  Legesse Garedew² 

[1] University of Gondar, Faculty of Veterinary Medicine, Gondar, Ethiopia;St. Paul’s Hospital Millennium Medical College, Department of Microbiology, Addis Ababa, Ethiopia;University of Montreal, Faculty of Veterinary Medicine, Montreal, Canada;Hawassa University, School of Veterinary Medicine, Awassa, Ethiopia;Max Planck Institute for Heart and Lung Research, Department III-Developmental Genetics, Bad Nauheim, Germany

关键词: Gondar;    susceptibility;    antimicrobials;    foods;    prevalence;    Listeria;   

Others  :  1212043
DOI  :  10.1186/s12866-015-0434-4

 received in 2014-09-25, accepted in 2015-05-01,  发布年份 2015

【 摘 要 】

Background

Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in Gondar town, Ethiopia. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Listeria species isolated from ready-to-eat food of animal origin from public dinning places in Gondar town, Ethiopia. A cross sectional study on ready-toeat foods of animal origin sampled from major supermarkets, butcher shops, pastry shops, restaurants and hotels was carried out. Culture, biochemical and sugar tests were conducted for listeria species identification and disc diffusion test was performed to study the antimicrobial susceptibility profiles of the isolates.

Results

Out of 384 food samples examined, 96 (25%) were positive for Listeria species. Listeria monocytogenes was detected in 24 (6.25%) of the samples. Listeria monocytogenes was isolated from cake, raw meat, ice cream, minced beef, fish, unpasteurized milk and pizza in that order from higher to lower rate. Assessment of antimicrobial susceptibility profile of L. monocytogenes revealed the presence of four multi-drug resistant isolates. The higher resistance rate was recorded for penicillin, nalidixic acid, tetracycline and chloramphenicol, in decreasing order. All L. monocytogenes identified in the current study were sensitive to amoxicillin, cephalothin, cloxacillin, sulfamethoxazole, gentamicin and vancomycin.

Conclusions

The presence of L. monocytogenes including drug resistant and multidrug resistant isolates in some ready-to-eat food items is an indicator of the presence of public health hazards to the consumer, particularly to the high-risk groups. Hence awareness creation on food safety and implementation of regulations about the use of drugs in humans and animals is strongly recommended.

【 授权许可】

   
2015 Garedew et al.; licensee BioMed Central.

【 参考文献 】

• [1]Van de Venter T. Prospects for the future: emerging problems-chemical/biological. Conference on International Food Trade Beyond 2000: Science–based Decision, Harmonization, Equivalence and Mutual Recognition Melbourne, Australia. FAO. 1999.1-20.
• [2]Vitas AI, Garcia-Jalón VA. Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). Int J Food Microbiol. 2004; 90:349-56.
• [3]Orsi RH, den Bakker HC, Wiedmann M. Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Medical Microbiol. 2011; 301:79-96.
• [4]Rocourt JC, Reilly A. Epidemiology of human Listeriosis and sea foods. Int J Food Microbiol. 2000; 62:197-209.
• [5]Teuber M. Spread of antibiotic resistance with foodborne pathogens. Cell Mol Life Sci. 1999; 56:755-63.
• [6]Central Statistical Investigatory: Statistical Report. Central Statistical Authority, Federal Democratic Republic of Ethiopia, Addis Ababa, Ethiopia; 2003.
• [7]Michael Thrusfield. 2007. Veterinary Epidemiology. 3rd Edition, Wiley-Blackwell publisher, Hoboken, New Jersey, USA; 151–208.
• [8]Rorvik LM, Caugant DA, Yndestad M. Contamination pattern of Listeria monocytogenes and other Listeria species in a salmon slaughterhouse and smoked salmon processing plant. Int J Food Microbiol. 1995; 25:19-27.
• [9]Microbiology of food and animal feeding stuffs-horizontal method for the detection and enumeration of Listeria monocytogenes. part1: Detection method ISO 11290–1. Intenational Organization for Standardization, Geneva, Switzerland; 1996.
• [10]Office International de Epizootics (OIE). Terrestrial manual, differentiation of Listeria species. OIE Terrestrial Manual, Paris, France; 2008, 1242.
• [11]Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45:493-496.
• [12]CLSI (Clinical and Laboratory Standards Institute): Performance for antimicrobial disk susceptibility tests; approved standard, 11th edition. CLSI document M02-A11. Wayne (PA), USA. 2012, 32(1):1–76.
• [13]Desalegn M, Bayleyegn M, Marie-Therese T, Kleer JC, Goetz H, Wondwossen AG. Occurrence and distribution of Listeria monocytogenes and other listeria species in ready-to-eat and raw neat products. Berl Munch Tierarztl Wochenschr. 2009; 122:20-24.
• [14]Firehiwot A. Prevalence and antimicrobial profile of Listeria monocytogenes in retail meat and dairy products in Addis Ababa and its surrounding towns, Ethiopia. MSc thesis, Addis Ababa University, Addis Ababa, Ethiopia; 2007.
• [15]Cordano AM, Rocourt J. Occurrence of Listeria monocytogenes in food in Chile. Int J food Microbiol. 2001; 70:175-178.
• [16]Yang HMA, Jaykus L, Morales RA. Consumer phase risk assessment for Listeria monocytogenes in Deli meats. Risk Analysis. 2006; 26(1):89.
• [17]Little CRD, Younas E, Louvois DJ. Microbiological quality of retail imported unprepared whole lettuce; a PHLS food working group study. J Food Prot. 1999; 62(4):325-328.
• [18]Baek S, Lim S, Lee D, Min K, Kim C. Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea. J food prot. 2000; 63(2):186-9.
• [19]Ryu CHIS, Inoue S, Kumagai S. The incidence of Listeria species in retail foods in Japan. Int J Food Microbiol. 1992; 16:157-60.
• [20]Gebretsadik S, Kassa T, Alemayehu H, Huruy K, Kebede N. Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia. J Infec Pub Hlth. 2011; 4(1):22-29.
• [21]Molla B, Yilma R, Alemayehu D. Listeria monocytogenes and other Listeria species in retail meat and milk products in Addis Ababa, Ethiopia. Ethio J Health Dev. 2004; 18(3):208-12.
• [22]Rocourt J, Bille J. Foodborne listeriosis. World Health Stat Q. 1997; 50:67-73.
• [23]Uhitil S, Jaksic S, Petrak T, Medic H, Gumhalter-Karolyi L. Prevalence of Listeria monocytogenes and other Listeria spp. in cakes in Croatia. Food contrl. 2004; 5:213-6.
• [24]Ferron P, Michard J. Distribution of Listeria species in confectioner’s pastries from western France: Comparison of enrichment methods. Int J Food Microbiol. 1993; 18:289-303.
• [25]Kozačinski L, Hadžiosmanovic’ M, Miokovic´ B, Njari B, Pranjic´ D, Cvrtila Ž. The meaning of Listeria monocytogenes in veterinary sanitary inspection. Vet Archiv. 2000; 70:7-13.
• [26]Rahimi E, Momtaz H, Sharifzadeh A, Behzadnia A, Ashtari MS, Zandi Esfahani S et al.. Prevalence and antimicrobial resistance of Listeria species isolated from traditional dairy products in Charar Mahal & Bakhtiary, Iran. Bulg J VetMed. 2012; 15(2):115-22.
• [27]Pintado CMBS, Oliveira A, Pampulha ME, Ferreira MASS. Prevalence and characterization of Listeria monocytogenes isolated from soft cheese. Food Microbiolo. 2005; 22:79-85.
• [28]Mahmodi MM. Occurrence of Listeria monocytogenes in raw milk and dairy products in Noorabad, Iran. J Anim Vet Advances. 2010; 9:16-9.3.
• [29]Navratilova P, Schlegelova J, Sustckova A, Napravnicova E, Lukasova J, Klimova E. Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains. Vet Med Czech. 2004; 49(7):243-52.
• [30]Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG et al.. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012; 18:268-281.
• [31]Sharma D, Sharma PK, Saharan BS, Malik A. Isolation, identification and antibiotic susceptibility profiling of antimicrobial resistant Listeria monocytogenes from dairy milk. Int J Microbial Res Techno. 2012; 1(1):1-4.