Cross-species chromosome painting tracks the independent origin of multiple sex chromosomes in two cofamiliar Erythrinidae fishes

BMC Evolutionary Biology

Luiz AC Bertollo⁴  Vladimir Trifonov³  Thomas Liehr²  Nadezda Kosyakova²  Juan A Marchal¹  Antonio Sánchez¹  Marcelo B Cioffi⁴ 

[1] Departamento de Biología Experimental, Universidad de Jaén, Jaén, Spain;Institute of Human Genetics, Jena University Hospital, Jena, Germany;Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia;Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil

关键词: Erythrinidae fish;    sex chromosome evolution;    fish cytogenetics;    microdissection;    chromosome painting;   

Others  :  1143669
DOI  :  10.1186/1471-2148-11-186

 received in 2011-04-11, accepted in 2011-06-30,  发布年份 2011

【 摘 要 】

Background

The Erythrinidae fish family is characterized by a large variation with respect to diploid chromosome numbers and sex-determining systems among its species, including two multiple X₁X₂Y sex systems in Hoplias malabaricus and Erythrinus erythrinus. At first, the occurrence of a same sex chromosome system within a family suggests that the sex chromosomes are correlated and originated from ancestral XY chromosomes that were either homomorphic or at an early stage of differentiation. To identify the origin and evolution of these X₁X₂Y sex chromosomes, we performed reciprocal cross-species FISH experiments with two sex-chromosome-specific probes designed from microdissected X₁ and Y chromosomes of H. malabaricus and E. erythrinus, respectively.

Results

Our results yield valuable information regarding the origin and evolution of these sex chromosome systems. Our data indicate that these sex chromosomes evolved independently in these two closed related Erythrinidae species. Different autosomes were first converted into a poorly differentiated XY sex pair in each species, and additional chromosomal rearrangements produced both X₁X₂Y sex systems that are currently present.

Conclusions

Our data provide new insights into the origin and evolution of sex chromosomes, which increases our knowledge about fish sex chromosome evolution.

【 授权许可】

   
2011 Cioffi et al; licensee BioMed Central Ltd.

【 图 表 】

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