Frequent detection of Streptococcus tigurinus in the human oral microbial flora by a specific 16S rRNA gene real-time TaqMan PCR

BMC Microbiology

Nagihan Bostanci⁴  Guido V Bloemberg¹  Patrick R Schmidlin³  Forouhar Mouttet¹  Reinhard Zbinden¹  Fatma Aras³  Andrea Zbinden² 

[1]Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland

[2]Institute of Medical Virology, University of Zurich, Winterthurerstrasse 190, Zurich, CH-8057, Switzerland

[3]Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland

[4]Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland

关键词: Oral microbiome;    Periodontitis;    Specific RT TaqMan PCR;    Streptococcus tigurinus;   

Others  :  1140483
DOI  :  10.1186/s12866-014-0231-5

 received in 2014-05-27, accepted in 2014-08-19,  发布年份 2014

【 摘 要 】

Background

Many bacteria causing systemic invasive infections originate from the oral cavity by entering the bloodstream. Recently, a novel pathogenic bacterium, Streptococcus tigurinus, was identified as causative agent of infective endocarditis, spondylodiscitis and meningitis. In this study, we sought to determine the prevalence of S. tigurinus in the human oral microbial flora and analyzed its association with periodontal disease or health.

Results

We developed a diagnostic highly sensitive and specific real-time TaqMan PCR assay for detection of S. tigurinus in clinical samples, based on the 16S rRNA gene. We analyzed saliva samples and subgingival plaque samples of a periodontally healthy control group (n = 26) and a periodontitis group (n = 25). Overall, S. tigurinus was detected in 27 (53%) out of 51 patients. There is no significant difference of the frequency of S. tigurinus detection by RT-PCR in the saliva and dental plaque samples in the two groups: in the control group, 14 (54%) out of 26 individuals had S. tigurinus either in the saliva samples and/or in the plaque samples; and in the periodontitis group, 13 (52%) out of 25 patients had S. tigurinus in the mouth samples, respectively (P = 0.895). The consumption of nicotine was no determining factor.

Conclusion

Although S. tigurinus was a frequently detected species of the human oral microbial flora, it was not associated with periodontal disease. Further investigations are required to determine whether S. tigurinus is a commensal or an opportunistic oral pathogen with a potential for development of invasive infections.

【 授权许可】

   
2014 Zbinden et al.; licensee BioMed Central Ltd

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Marsh PD: Are dental diseases examples of ecological catastrophes? Microbiology 2003, 149(Pt 2):279-294.
• [2]Albandar JM: Underestimation of periodontitis in NHANES surveys. J Periodontol 2011, 82(3):337-341.
• [3]Konig J, Holtfreter B, Kocher T: Periodontal health in Europe: future trends based on treatment needs and the provision of periodontal services–position paper 1. Eur J Dent Educ 2010, 14(Suppl 1):4-24.
• [4]Marcenes W, Kassebaum NJ, Bernabe E, Flaxman A, Naghavi M, Lopez A, Murray CJ: Global burden of oral conditions in 1990–2010: a systematic analysis. J Dent Res 2013, 92(7):592-597.
• [5]Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE: Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005, 43(11):5721-5732.
• [6]Papapanou PN, Behle JH, Kebschull M, Celenti R, Wolf DL, Handfield M, Pavlidis P, Demmer RT: Subgingival bacterial colonization profiles correlate with gingival tissue gene expression. BMC Microbiol 2009, 9:221. BioMed Central Full Text
• [7]Socransky SS, Haffajee AD: Implications of periodontal microbiology for the treatment of periodontal infections. Compt Rendus Geosci 1994, 18:S684-S685. 688–693; quiz S714-687
• [8]Lalla E, Papapanou PN: Diabetes mellitus and periodontitis: a tale of two common interrelated diseases. Nat Rev Endocrinol 2011, 7(12):738-748.
• [9]Beck JD, Offenbacher S: Systemic effects of periodontitis: epidemiology of periodontal disease and cardiovascular disease. J Periodontol 2005, 76(11 Suppl):2089-2100.
• [10]Spellerberg B, Brandt C: Streptococcus. In Manual of clinical microbiology. Volume 1. 10th edition. Edited by Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW. Washington, DC: ASM Press; 2011:331–349.
• [11]Zbinden A, Mueller NJ, Tarr PE, Sproer C, Keller PM, Bloemberg G: Streptococcus tigurinussp. nov., isolated from blood of patients with endocarditis, meningitis and spondylodiscitis.Int J Syst Evol Microbiol 2012, 62(Pt 12):2941–2945.
• [12]Zbinden A, Mueller NJ, Tarr PE, Eich G, Schulthess B, Bahlmann AS, Keller PM, Bloemberg GV: Streptococcus tigurinus, a novel member of theStreptococcus mitisgroup, causes invasive infections.J Clin Microbiol 2012, 50(9):2969–2973.
• [13]Zbinden A, Quiblier C, Hernandez D, Herzog K, Bodler P, Senn MM, Gizard Y, Schrenzel J, François P: Characterization ofStreptococcus tigurinussmall-colony variants causing prosthetic joint infection by comparative whole-genome analyses.J Clin Microbiol 2014, 52(2):467–474.
• [14]Veloso TR, Zbinden A, Andreoni F, Giddey M, Vouillamoz J, Moreillon P, Zinkernagel AS, Entenza JM: Streptococcus tigurinusis highly virulent in a rat model of experimental endocarditis.Int J Med Microbiol 2013, 303(8):498–504.
• [15]Lo Frisco C, Cutler R, Bramson JB: Periodontal screening and recording: perceptions and effects on practice. J Am Dent Assoc 1993, 124(7):226-229. 231–232
• [16]Cutress TW, Ainamo J, Sardo-Infirri J: The community periodontal index of treatment needs (CPITN) procedure for population groups and individuals. Int Dent J 1987, 37(4):222-233.
• [17]Belibasakis GN, Schmidlin PR, Sahrmann P: Molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette. APMIS 2014, 122(4):347-352.
• [18]Deshpande RG, Khan M, Genco CA: Invasion strategies of the oral pathogenPorphyromonas gingivalis: implications for cardiovascular disease.Invasion Metastasis 1998, 18(2):57–69.
• [19]Kinane DF, Bartold PM: Clinical relevance of the host responses of periodontitis. Periodontol 2000 2007, 43:278-293.
• [20]Hamedi M, Belibasakis GN, Cruchley AT, Rangarajan M, Curtis MA, Bostanci N: Porphyromonas gingivalisculture supernatants differentially regulate interleukin-1beta and interleukin-18 in human monocytic cells.Cytokine 2009, 45(2):99–104.
• [21]Li J, Helmerhorst EJ, Leone CW, Troxler RF, Yaskell T, Haffajee AD, Socransky SS, Oppenheim FG: Identification of early microbial colonizers in human dental biofilm. J Appl Microbiol 2004, 97(6):1311-1318.
• [22]Sliepen I, Van Damme J, Van Essche M, Loozen G, Quirynen M, Teughels W: Microbial interactions influence inflammatory host cell responses. J Dent Res 2009, 88(11):1026-1030.
• [23]Ennibi OK, Benrachadi L, Bouziane A, Haubek D, Poulsen K: The highly leukotoxic JP2 clone ofAggregatibacter actinomycetemcomitansin localized and generalized forms of aggressive periodontitis.Acta Odontol Scand 2009, 70(4):318–322.
• [24]Gizard Y, Zbinden A, Schrenzel J, François P: Whole-genome sequences ofStreptococcus tigurinustype strain AZ_3a andS. tigurinus1366, a strain causing prosthetic joint infection.Genome Announc 2013, 1(2):e00210–e00212.
• [25]Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner AC, Yu WH, Lakshmanan A, Wade WG: The human oral microbiome. J Bacteriol 2010, 192(19):5002-5017.
• [26]Belibasakis GN, Ozturk VO, Emingil G, Bostanci N: Synergistetescluster A in saliva is associated with periodontitis.J Periodontal Res 2013, 48(6):727–732.