Transcriptional profiles of Burkholderia pseudomallei reveal the direct and indirect roles of Sigma E under oxidative stress conditions

BMC Genomics

Sunee Korbsrisate⁴  Patrick Tan²  Richard W Titball⁵  Paiboon Vattanaviboon³  Claudia M Hemsley⁵  Wen Fong Ooi¹  Niramol Juntawieng⁴  Catherine Ong²  Siroj Jitprasutwit⁴ 

[1] Genome Institute of Singapore, Singapore 138672, Singapore;DSO National Laboratories, Singapore 117510, Singapore;Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand;Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;Bioscience, Geoffrey Pope Building, University of Exeter, Devon EX4 4QD, UK

关键词: Oxidative stress;    SpeG;    Sigma E;    Transcription profile;    B. pseudomallei;   

Others  :  1140553
DOI  :  10.1186/1471-2164-15-787

 received in 2014-03-29, accepted in 2014-09-08,  发布年份 2014

【 摘 要 】

Background

Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative bacterium widely distributed in soil and water in endemic areas. This soil saprophyte can survive harsh environmental conditions, even in soils where herbicides (containing superoxide generators) are abundant. Sigma factor E (σ^E) is a key regulator of extra-cytoplasmic stress response in Gram-negative bacteria. In this study, we identified the B. pseudomallei σ^E regulon and characterized the indirect role that σ^E plays in the regulation of spermidine, contributing to the successful survival of B. pseudomallei in stressful environments.

Results

Changes in the global transcriptional profiles of B. pseudomallei wild type and σ^E mutant under physiological and oxidative stress (hydrogen peroxide) conditions were determined. We identified 307 up-regulated genes under oxidative stress condition. Comparison of the transcriptional profiles of B. pseudomallei wild type and σ^E mutant under control or oxidative stress conditions identified 85 oxidative-responsive genes regulated by σ^E, including genes involved in cell membrane repair, maintenance of protein folding and oxidative stress response and potential virulence factors such as a type VI secretion system (T6SS). Importantly, we identified that the speG gene, encoding spermidine-acetyltransferase, is a novel member of the B. pseudomallei σ^E regulon. The expression of speG was regulated by σ^E, implying that σ^E plays an indirect role in the regulation of physiological level of spermidine to protect the bacteria during oxidative stress.

Conclusion

This study identified B. pseudomallei genes directly regulated by σ^E in response to oxidative stress and revealed the indirect role of σ^E in the regulation of the polyamine spermidine (via regulation of speG) for bacterial cell protection during oxidative stress. This study provides new insights into the regulatory mechanisms by which σ^E contributes to the survival of B. pseudomallei under stressful conditions.

【 授权许可】

   
2014 Jitprasutwit et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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