Increased CCL19 and CCL21 levels promote fibroblast ossification in ankylosing spondylitis hip ligament tissue

BMC Musculoskeletal Disorders

Zou Yu Ming³  Tong Wen Wen³  Xu Wei Dong³  Yang Sheng Sheng¹  Miao Ming Yong¹  Zhou Jian Sheng¹  Li Da He³  Yang Qin² 

[1]Department of Biochemistry and Molecular Biology, Second Military Medical University, Xiangyin Road 800, Shanghai 200433, Yangpu District, P. R. China

[2]School of Kinesiology, Shanghai University of Sport, Hengren Road 200, Shanghai 200438, Yangpu District, P. R. China

[3]Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Changhai Road 168, Shanghai 200433, Yangpu district, P. R. China

关键词: Ossification;    Fibroblast;    Ankylosing spondylitis;    CCL21;    CCL19;   

Others  :  1122060
DOI  :  10.1186/1471-2474-15-316

 received in 2014-06-30, accepted in 2014-08-27,  发布年份 2014

【 摘 要 】

Background

It is well-documented that both chemokine (C-C motif) ligand 19 (CCL19) and 21 (CCL21) mediate cell migration and angiogenesis in many diseases. However, these ligands’ precise pathological role in ankylosing spondylitis (AS) has not been elucidated. The objective of this study was to examine the expression of CCL19 and CCL21 (CCL19/CCL21) in AS hip ligament tissue (LT) and determine their pathological functions.

Methods

The expression levels of CCL19, CCL21 and their receptor CCR7 in AS (n = 31) and osteoarthritis (OA, n = 21) LT were analyzed via real-time polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC). The expression of CCL19, CCL21 and CCR7 in AS ligament fibroblasts was also detected. The proliferation of ligament fibroblasts was measured via a cell counting kit-8 (CCK8) assay after exogenous CCL19/CCL21 treatment. Additionally, the role of CCL19/CCL21 in osteogenesis was evaluated via RT-PCR and enzyme-linked immunosorbent assay (ELISA) in individual AS fibroblast cultures. Furthermore, the expression of the bone markers alkaline phosphatase (ALP), osteocalcin (OCN), collagenase I (COL1), integrin-binding sialoprotein (IBSP) and the key regulators runt-related transcription factor-2 (Runx-2) and osterix were investigated. Moreover, the CCL19/CCL21 levels in serum and LT were measured via ELISA.

Results

The mRNA levels of CCL19/CCL21 in AS hip LT were significantly higher than that in OA LT, and IHC analysis revealed a similar result. Exogenous CCL19/CCL21 treatment did not affect the proliferation of ligament fibroblasts but significantly up-regulated the expression of bone markers, including ALP and OCN, and the key regulators Runx-2 and osterix. In addition, the serum levels of CCL19/CCL21 were apparently elevated in AS patients compared to healthy controls (HC), and the expression of the two chemokines correlated significantly in AS patients.

Conclusions

CCL19 and CCL21, two chemokines displaying significantly associated expression in serum, indicating a synergistic effect on AS pathogenesis, may function as promoters of ligament ossification in AS patients.

【 授权许可】

   
2014 Qin et al.; licensee BioMed Central Ltd.

【 图 表 】

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