Body composition among Sri Lankan infants by 18O dilution method and the validity of anthropometric equations to predict body fat against 18O dilution

BMC Pediatrics

William Wai-Lun Wong¹  Sujeewa Amarasena²  Chandrani Liyanage²  Manjula Hettiarachchi²  Thushari Bandara² 

[1] USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA;Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka

关键词: Mid upper-arm circumference;    Skinfold thickness;    18O dilution;    Isotope ratio mass spectrometry;    Fat free mass;    Fat mass;    Total body water;   

Others  :  1203850
DOI  :  10.1186/s12887-015-0371-2

 received in 2014-12-01, accepted in 2015-04-27,  发布年份 2015

【 摘 要 】

Background

Body composition indicators provide a better guidance for growth and nutritional status of the infants. This study was designed to (1) measure the body composition of the Sri Lankan infants using a reference method, the ¹⁸O dilution method; (2) calculate the body fat content of the infants using published skinfold prediction equations; and (3) evaluate the applicability of the skinfold equations to predict body fat among Sri Lankan infants against the ¹⁸O dilution method.

Methods

Twenty five healthy, exclusively breast-fed infants were randomly recruited at well-baby clinics, for this cross-sectional study. Body composition was measured using ¹⁸O dilution. Infant body weight, length, skinfold thicknesses and mid upper-arm circumference were measured using standard procedures. The Bland and Atlman pair-wise comparison method was used to evaluate the agreement of body fat generated using the anthropometric prediction equations against the ¹⁸O dilution values as the reference.

Results

Mean (SD) body weight and length of the infants were 6.5 kg (0.9) and 64.7 cm (2.8) respectively. Mean total body water, fat free mass, fat mass and % fat mass as measured by ¹⁸O dilution method were 58.8% (5.0), 4.6 kg (0.8), 1.9 (0.5) and 29.5% (6.1). Total body water and fat free mass were significantly higher in boys when compared to girls. With the exception of three prediction equations (Bandana et al., Goran et al. and Durnin and Wormsley), most of the other commonly used anthropometry-based prediction equations yielded a bias which was not constant but a function of the % fat mass.

Conclusions

Body composition of Sri Lankan infants is comparable to the normative data available from the industrialized countries. Most of the commonly used anthropometric prediction equations generated a bias which varies with the size of the body fat. Only three prediction equations (Bandana, Goran, Durnin & Wormsley) yield a constant bias. The Durnin & Wormsely equation showed the smallest bias when compared to the ¹⁸O dilution values with the narrowest limits of agreement. Accuracy of some of the prediction equations is a function of gender.

【 授权许可】

   
2015 Bandara et al.; licensee BioMed Central.

【 参考文献 】

• [1]IAEA human health series No.22. Body composition assessment from birth to two years of age. IAEA, Vienna; 2013.
• [2]Kensara OA, Wooton SA, Phillips DI, Patel M, Hoffman DJ, Jackson AA et al.. Substrate energy metabolism and metabolic risk factors for cardiovascular disease in relation to fetal growth and adult body composition. Am J Physiol Endocrinol Metab. 2006; 291 Supple 2:365-71.
• [3]Keys A, Brozek J. Body fat in adult men. Physiol Rev. 1953; 33:245-325.
• [4]Wong WW, Lee LS, Klein PD. Deuterium and oxygen-18 measurements on microliter samples of urine, plasma, saliva, and human milk. Am J Clin Nutr. 1987; 45:905-13.
• [5]Wong WW, Klein PD, Parr RM, Clements SA. Interlaboratory analysis of reference water samples enriched with deuterium and oxygen-18. Appl Radiat Isot. 1993; 44:561-6.
• [6]Introduction to body composition assessment using the deuterium dilution technique with analysis of saliva samples by fourier transform infrared spectrometry, IAEA Human Health Series No. 12. IAEA, Vienna; 2011.
• [7]Introduction to body composition assessment using the deuterium dilution technique with analysis of urine samples by isotope ratio mass spectrometry, IAEA Human Health Series No. 13. IAEA, Vienna; 2011.
• [8]Schoeller DA, Santen E, Peterson DW, Dietz W, Jaspan J, Klein PD. Total body water measurement in humans with 18O and 2H labeled water. Am J Clin Nutri. 1980; 33:2286-693.
• [9]Butte NF, Hopkinson JM, Wong WW, Smith EO, Ellis KJ. Body composition during the first 2 years of life: an updated reference. Pediatr Res. 2000; 47:578-85.
• [10]Lohman TG, Roche AF, Martorell R. Anthropometric standardization reference manual. Human kinetics, Champaign, IL; 1988.
• [11]Gibson RS. Principles of nutritional assessment. Oxford University Press, New York; 1990.
• [12]Sen B, Bose K, Shaikh S, Mahalanabis D. Prediction equations for body-fat percentage in Indian infants and young children using skinfold thickness and mid-arm circumference. J Health Popul Nutr. 2010; 3:221-9.
• [13]Shaikh S, Mahalanabis D. Empirically derived new equations for calculating body fat percentage based on skinfold thickness and midarm circumference in preschool Indian children. Am J Human Biol. 2004; 16:278-88.
• [14]Hoffman DJ, Toro-Ramos T, Sawaya AL, Roberts SB, Rondo P. Estimating total body fat using a skinfold prediction equation in Brazilian children. Ann Human Biol. 2012; 2:156-60.
• [15]Goran MI, Driscoll P, Johnson R, Nagy TR, Hunter G. Cross calibration of body-composition techniques against dual-energy Xray absorptiometry in young children. Am J Clin Nutr. 1996; 63:299-305.
• [16]Slaughter MH, Lohman TG, Boileau RA, Horswill CA, Stillman RJ, Van Loan MD et al.. Skinfold equations for estimation of body fatness in children and youth. Hum Biol. 1988; 60:709-23.
• [17]Durnin JVGA, Rahaman MM. The assessment of the amount of fat in the human body from measurements of skinfold thickness. Br J Nutr. 1967; 21:681-9.
• [18]Brook CGD. Determination of body composition of children from skinfold measurements. Arch Dis Child. 1971; 46:182-4.
• [19]Yuan T, Xin C, Sun Y. Study of body composition and obesity definition of children and adolescents. Chinese J Prevent Med. 1987; 21:172-3.
• [20]Liu NH, Hourihane JO, Kenny L, Kiely M, Irvine AD, Murray DM. Comparison of body fat estimation using skinfold thickness measurement and simultaneous air displacement plethysmography at 8 weeks. Pediatr Res. 2010; 68:208-8.
• [21]Deurenberg P, Pieters JJL, Hautvast JGAJ. The assessment of the body fat percentage by skinfold thickness measurements in childhood and young adolescence. Br J Nutr. 1990; 63:293-303.
• [22]Durnin JVGA, Womersley J. Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16–72 years. Br J Nutr. 1974; 32:77-97.
• [23]Sloan AW, Burt JJ, Blyth CS. Estimation of body fat in young women. J Appl Physiol. 1962; 17:967-70.
• [24]Altman DG, Bland JM. Measurement in Medicine: the analysis of method comparison studies. The Statistician. 1983; 32:307-17.
• [25]Wells JC, Chomtho S, Fewtrell MS. Programming of body composition by early growth and nutrition. Proc Nutr Soc. 2007; 66:423-34.
• [26]Schmelzle HR, Fusch C. Body fat in neonates and young infants: validation of skinfold thickness versus dual-energy X-ray absorptiometry1–3. Am J Clin Nutr. 2002; 76:1096-100.
• [27]Ellis KJ, Yao M, Shypailo RJ, Urlando A, Wong WW, Heird WC. Body-composition assessment in infancy: air-displacement plethysmography compared with a reference 4-compartment model. Am J Clin Nutr. 2007; 85:90-5.
• [28]Assessment of body composition and total energy expenditure in humans using stable isotope techniques, IAEA Human Health Series No. 3. IAEA, Vienna; 2009.
• [29]Wells JCK, Fewtrell MS, Davies PSW, Williams JE, Coward WA, Cole TJ. Prediction of total body water in infants and children. Arch Dis Child. 2005; 90:965-71.
• [30]Friis-Hansen BJ, Holiday M, Stapleton T, Wallagce WM. Total body water in children. Pediatrics. 1951; 7:321-7.
• [31]Fomon SJ, Haschke F, Ziegler EE, Nelson SE. Body composition of reference children from birth to age 10 years. Am J Clin Nutr. 1982; 35:1169-75.
• [32]Fields DA, Janet M. Gilchrist, Patrick M. Catalano, Giannì ML, et al. Longitudinal body composition data in exclusively breast-fed infants: A multicenter study. Obesity. 2011, doi:10.1038/oby.2011.11.