Analysis of Y-P30/Dermcidin expression and properties of the Y-P30 peptide

BMC Research Notes

Michael R Kreutz⁴  Ana C Zenclussen³  Petra Wahle²  Tarek El-Mousleh³  Tamar Macharadze⁴  Janine R Neumann²  Corinna Borutzki⁴  Anne Schumacher³  Marina Mikhaylova¹ 

[1] Cell Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands;Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University, Bochum, Germany;Department of Experimental Obstetrics and Gynaecology, Medical Faculty, Otto-von-Guericke University, Magdeburg 39120, Germany;RG Neuroplasticity, Leibniz-Institute for Neurobiology, Magdeburg 39118, Germany

关键词: PBMCs;    Dermcidin Y-P30;    Syndecans;    Placenta;   

Others  :  826522
DOI  :  10.1186/1756-0500-7-400

 received in 2013-10-16, accepted in 2014-06-20,  发布年份 2014

【 摘 要 】

Background

The survival promoting peptide Y-P30 has a variety of neuritogenic and neuroprotective effects in vitro and in vivo. In previous work we reported the expression of Y-P30/dermcidin in maternal peripheral blood mononuclear cells (PBMCs) and the transport of the protein to the fetal brain. In this study we analyzed hormonal regulation of Y-P30 in human immune cells and expression of Y-P30 in the placenta. We further studied the stability and secretion of the Y-P30 peptide.

Results

We found indications that Y-P30 might be produced in human placenta. The Y-P30 mRNA was rarely found in isolated human PBMCs and alpha-feto-protein, human chorionic gonadotropin as well as estradiol combined with progesterone could not induce Y-P30 expression. Y-P30 was found to be extraordinarily stable; therefore, contamination with the peptide and the Y-P30/Dermcidin precursor mRNA is a serious concern in experiments looking at the expression of Y-P30/Dermcidin. In cultured cell lines and primary neurons we found that Y-P30 could be released, but neuronal uptake of Y-P30 was not observed.

Conclusions

Our data suggest that a source of Y-P30 apart from eccrine glands might be the placenta. The peptide can be secreted together with the signaling peptide and it might reach the fetal brain where it can exert its neuritogenic functions by binding to neuronal membranes.

【 授权许可】

   
2014 Mikhaylova et al.; licensee BioMed Central Ltd.

【 图 表 】

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