Cross-sectional study of hepatitis B virus infection in rural communities, Central African Republic

BMC Infectious Diseases

Alain Le Faou³  Claude P Muller²  Alexandre Manirakiza¹  Aubin Béré⁴  Judith M Hübschen²  Ulrich Vickos⁴  Narcisse P Komas⁴ 

[1] Epidemiology service, Institut Pasteur de Bangui, Bangui, Central African Republic;Institute of Immunology, Centre de Recherche Publique de la Santé / Laboratoire National de Santé, Luxembourg, Luxembourg;New affiliation: EA 3452 CITHEFOR ; Faculté de Pharmacie, Université de Lorraine, Nancy, France;Viral Hepatitis Laboratory, Institut Pasteur de Bangui, PO Box 923, Bangui, Central African Republic

关键词: HBV genotyping;    Rural community;    HBV prevalence;    HBV risk factors;    Central African Republic;    HBV;    Hepatitis B virus;   

Others  :  1147518
DOI  :  10.1186/1471-2334-13-286

 received in 2013-02-18, accepted in 2013-06-18,  发布年份 2013

【 摘 要 】

Background

As most data on hepatitis in resource-poor countries relate to urban communities, surveys in the rural environment are necessary to determine the ‘true’ prevalence of these viral infections. We undertook a survey to determine the prevalence of hepatitis B virus (HBV) infection in an apparently healthy rural population in the Central African Republic (CAR).

Methods

The cross-sectional study was based on dried blood spots (DBS) from 273 people recruited in four prefectures (Lobaye, Nana-Mambéré, Ouham and Ouaka). Eluates from DBS were tested with commercial ELISA kits to detect markers of HBV infection. DBS were directly used for DNA extraction, followed by PCR and genotyping based on preS/S gene sequences.

Results

The overall prevalence of HBc antibodies was 27.1% (Lobaye 29%, Nana-Mambéré 28%, Ouaka 29% and Ouham 23%) and that of HBsAg was 10.6% (Lobaye 9%, Nana-Mambéré 9%, Ouaka 19% and Ouham 8%), with no statistically significant difference among the surveyed communities. Nineteen sequences obtained from 74 anti-HBc-positive patients all belonged to genotype E. Risk factor analysis of HBV infection pointed to sexual transmission of the virus.

Conclusion

The prevalence of HBV is high in rural communities in the CAR and comparable to that observed in urban areas. In addition, genotype E is prevalent in these areas. These findings underline the importance of instituting a programme of active HBV surveillance and vaccination of the population.

【 授权许可】

   
2013 Komas et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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