Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour

BMC Microbiology

Valentina Rosu¹  Stefania Zanetti²  Leonardo Antonio Sechi²  Andrea Cossu² 

[1] Experimental Zooprophylactic Institute of Sardinia, Department of Nuoro, Nuoro, Italy;Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy

关键词: macrophage infection;    simulated intra-phagosomal multi-stress;    DNA-microarray;    functional genomics;    Mycobacterium avium subsp. paratuberculosis;   

Others  :  1221928
DOI  :  10.1186/1471-2180-12-87

 received in 2012-01-14, accepted in 2012-05-30,  发布年份 2012

【 摘 要 】

Background

Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene expression during infection of human host as well as the identification of its immunogenic and/or virulence factors for the development of appropriate diagnostic and therapeutic tools.

Results

In order to characterize MAP's transcriptome during macrophage infection, we analyzed for the first time the whole gene expression of a human derived strain of MAP in simulated intraphagosomal conditions and after intracellular infection of the human macrophage cell line THP-1 by using the DNA-microarray technology. Results showed that MAP shifts its transcriptome to an adaptive metabolism for an anoxic environment and nutrient starvation. It up-regulates several response factors to oxidative stress or intracellular conditions and allows, in terms of transcription, a passive surface peptidoglycan spoliation within the macrophage along with an intensification of the anabolic activity for lipidic membrane structures.

Conclusions

These results indicate a possible interactive system between MAP and its host cell based on the internal mimicry unlike other intracellular pathogens, bringing new hypothesis in the virulence and pathogenicity of MAP and its importance in human health.

【 授权许可】

   
2012 Cossu et al.; licensee BioMed Central Ltd.

【 图 表 】

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