Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line

BMC Developmental Biology

Shawn M Burgess⁴  Brant M Weinstein²  Shin-ichi Higashijima¹  Chie Satou¹  Kenna M Shaw³  Lisha Xu⁴  Seth Zonies⁴  Jessica Sheehy⁴  Jennifer Idol⁴  Abdel Elkahloun⁴  Aranza Torrado⁵  John Bradsher⁵  Viviana E Gallardo⁴  Martine Behra⁵ 

[1] National Institutes of Natural Sciences, Okazaki Institute for Integrative Biology, Okazaki, Aichi 444-8787 Japan;National Institute of Child Health and Human Development, Bethesda, MD 20892, USA;National Cancer Institute, Bethesda, MD 20892, USA;National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA;Department of Anatomy and Neurobiology, Medical school, University of Puerto Rico, PR 00936, USA

关键词: Tnk1bp1;    microarrays;    accessory cells;    supporting cells;    zebrafish;    lateral line;    progenitor cells;    hair cells;    Regeneration;   

Others  :  1086769
DOI  :  10.1186/1471-213X-12-6

 received in 2011-10-27, accepted in 2012-01-24,  发布年份 2012

【 摘 要 】

Background

Because of the structural and molecular similarities between the two systems, the lateral line, a fish and amphibian specific sensory organ, has been widely used in zebrafish as a model to study the development/biology of neuroepithelia of the inner ear. Both organs have hair cells, which are the mechanoreceptor cells, and supporting cells providing other functions to the epithelium. In most vertebrates (excluding mammals), supporting cells comprise a pool of progenitors that replace damaged or dead hair cells. However, the lack of regenerative capacity in mammals is the single leading cause for acquired hearing disorders in humans.

Results

In an effort to understand the regenerative process of hair cells in fish, we characterized and cloned an egfp transgenic stable fish line that trapped tnks1bp1, a highly conserved gene that has been implicated in the maintenance of telomeres' length. We then used this Tg(tnks1bp1:EGFP) line in a FACsorting strategy combined with microarrays to identify new molecular markers for supporting cells.

Conclusions

We present a Tg(tnks1bp1:EGFP) stable transgenic line, which we used to establish a transcriptional profile of supporting cells in the zebrafish lateral line. Therefore we are providing a new set of markers specific for supporting cells as well as candidates for functional analysis of this important cell type. This will prove to be a valuable tool for the study of regeneration in the lateral line of zebrafish in particular and for regeneration of neuroepithelia in general.

【 授权许可】

   
2012 Behra et al; licensee BioMed Central Ltd.

【 图 表 】

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