Positive selection in glycolysis among Australasian stick insects

BMC Evolutionary Biology

Thomas R Buckley²  Richard D Newcomb⁴  Brent J Sinclair¹  Geoffrey Thomson⁵  Alice B Dennis²  Luke T Dunning³ 

[1] Department of Biology, The University of Western Ontario, London, ON, Canada N6G 1L3;Allan Wilson Centre for Molecular Ecology and Evolution, Auckland, New Zealand;Imperial College London, Silwood Park Campus, Buckhurst Road, SL5 7PY, Ascot, Berks, UK;The New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand;School of Biological Sciences, University of Auckland, Auckland, New Zealand

关键词: Phosphoglucose isomerase;    Oxidative stress;    Lipid biosynthesis;    Molecular evolution;    Lanceocercata;   

Others  :  1085796
DOI  :  10.1186/1471-2148-13-215

 received in 2013-05-02, accepted in 2013-09-23,  发布年份 2013

【 摘 要 】

Background

The glycolytic pathway is central to cellular energy production. Selection on individual enzymes within glycolysis, particularly phosphoglucose isomerase (Pgi), has been associated with metabolic performance in numerous organisms. Nonetheless, how whole energy-producing pathways evolve to allow organisms to thrive in different environments and adopt new lifestyles remains little explored. The Lanceocercata radiation of Australasian stick insects includes transitions from tropical to temperate climates, lowland to alpine habitats, and winged to wingless forms. This permits a broad investigation to determine which steps within glycolysis and what sites within enzymes are the targets of positive selection. To address these questions we obtained transcript sequences from seven core glycolysis enzymes, including two Pgi paralogues, from 29 Lanceocercata species.

Results

Using maximum likelihood methods a signature of positive selection was inferred in two core glycolysis enzymes. Pgi and Glyceraldehyde 3-phosphate dehydrogenase (Gaphd) genes both encode enzymes linking glycolysis to the pentose phosphate pathway. Positive selection among Pgi paralogues and orthologues predominately targets amino acids with residues exposed to the protein’s surface, where changes in physical properties may alter enzyme performance.

Conclusion

Our results suggest that, for Lancerocercata stick insects, adaptation to new stressful lifestyles requires a balance between maintaining cellular energy production, efficiently exploiting different energy storage pools and compensating for stress-induced oxidative damage.

【 授权许可】

   
2013 Dunning et al.; licensee BioMed Central Ltd.

【 图 表 】

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