Lactobacillus fermentation enhances the inhibitory effect of Hwangryun-haedok-tang in an ovariectomy-induced bone loss

BMC Complementary and Alternative Medicine

Jin Yeul Ma³  Dong-Hyun Seo¹  Han Sung Kim¹  Chang-Won Cho²  Kwang Jin Lee³  Hyunil Ha³  Taesoo Kim³  Ki-Shuk Shim³ 

[1] Yonsei-Fraunhofer Medical Device Lab, Yonsei University, Wonju Gangwon, 220-710, South Korea;Regional Food Industry Research Group, Korea Food Research Institute, Sungnam 463-746, South Korea;KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, South Korea

关键词: Ovariectomy;    RANKL;    Osteoclastogenesis;    Lactobacillus curvatus;    Hwangryun-haedok-tang;   

Others  :  1229958
DOI  :  10.1186/1472-6882-13-106

 received in 2012-09-18, accepted in 2013-05-13,  发布年份 2013

【 摘 要 】

Background

Hwangryun-haedok-tang (HRT) is traditional herbal medicine used to treat inflammatory-related diseases in Asia. However, its effect on osteoclastogenesis and bone loss is still unknown. In this study, we evaluated the effect of HRT and its fermented product (fHRT) on the receptor activator for the nuclear factor-κB ligand-induced osteoclastogenesis using murine bone marrow-derived macrophages and postmenopausal bone loss using an ovariectomy (OVX) rat model.

Methods

Tartrate resistant acid phosphatase (TRAP) staining was employed to evaluate osteoclast formation. mRNA level of transcription factor and protein levels of signaling molecules were determined by real-time quantitative polymerase chain reaction and Western blot analysis, respectively. Effect of HRT or fHRT on OVX-induced bone loss was evaluated using OVX rats orally administered HRT, or fHRT with 300 mg/kg for 12 weeks. Micro-CT analysis of femora was performed to analyze bone parameter.

Results

HRT or fHRT treatment significantly decreased TRAP activity and the number of TRAP positive multinuclear cells on osteoclastogenesis. Interestingly, these inhibitory effects of HRT were enhanced by fermentation. Furthermore, fHRT significantly inhibited mRNA and protein expression of nuclear factor of activated T cells cytoplasmic 1, which leads to down-regulation of NFATc1-regulated mRNA expressions such as TRAP, the d2 isoform of vacuolar ATPase V(0) domain, and cathepsin K. Administration of fHRT significantly inhibited the decrease of bone mineral density, and improved bone parameter of femora more than that of HRT and vehicle in OVX rats.

Conclusions

This study demonstrated that lactic bacterial fermentation fortifies the inhibitory effect of HRT on osteoclastogenesis and bone loss. These results suggest that fermented HRT might have the beneficial potential on osteoporosis by inhibiting osteoclastogenesis.

【 授权许可】

   
2013 Shim et al.; licensee BioMed Central Ltd.

【 图 表 】

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