Preliminary study of the antioxidant properties of flowers and roots of Pyrostegia venusta (Ker Gawl) Miers

BMC Complementary and Alternative Medicine

Vinod Singh²  Avnish Kumar¹  Sarika Amdekar²  Purabi Roy² 

[1] Department of Biotechnology, School of Life Sciences, Dr. B. R. Ambedkar University, Agra-282002, Uttar Pradesh, India;Department of Microbiology, Barkatullah University, Hoshangabad Road, Habibganj, Bhopal, 462024, Madhya Pradesh, India

关键词: Pyrostegia venusta;    Flavonoids;    DPPH;    Antioxidants;   

Others  :  1232979
DOI  :  10.1186/1472-6882-11-69

 received in 2011-04-22, accepted in 2011-08-23,  发布年份 2011

【 摘 要 】

Background

Free radical stress leads to tissue injury and can eventually to arthritis, atherosclerosis, diabetes mellitus, neurodegenerative diseases and carcinogenesis. Several studies are ongoing worldwide to find natural antioxidants of plant origin. We assessed the in-vitro antioxidant activities and screened the phytochemical constituents of methanolic extracts of Pyrostegia venusta (Ker Gawl) Miers.

Methods

We evaluated the antioxidant potential and phytochemical constituents of P. venusta using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Gas chromatography-mass spectroscopy (GC-MS) studies were also undertaken to assess the phytochemical composition of the flower extracts.

Results

Phytochemical analyses revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. The reducing ability of both extracts was in the range (in μm Fe(II)/g) of 112.49-3046.98 compared with butylated hydroxytoluene (BHT; 63.56 ± 2.62), catechin (972.02 ± 0.72 μm) and quercetin 3208.27 ± 31.29. A significant inhibitory effect of extracts of flowers (IC₅₀ = 0.018 ± 0.69 mg/ml) and roots (IC₅₀ = 0.026 ± 0.94 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extracts of flowers (95%) and roots (94%) on DPPH radicals was comparable with that of ascorbic acid (98.9%) and BHT (97.6%). GC-MS study revealed the presence of myoinositol, hexadecanoic acid, linoleic acid, palmitic acid and oleic acid in the flower extracts.

Conclusion

These data suggest that P. venusta is a natural source of antioxidants. The extracts of flowers and roots of P. venusta contain significant amounts of phytochemicals with antioxidative properties and could serve as inhibitors or scavengers of free radicals. P. venusta could be exploited as a potential source for plant-based pharmaceutical products. These results could form a sound basis for further investigation in the potential discovery of new natural bioactive compounds.

【 授权许可】

   
2011 Roy et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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