A medium density genetic map and QTL for behavioral and production traits in Japanese quail

BMC Genomics

Elisabeth Le Bihan-Duval³  Christine Leterrier¹  Catherine Beaumont³  Francis Minvielle²  Maria Ines Fariello⁵  Alain Vignal⁵  Olivier Bouchez⁵  David Gourichon⁶  Aline Bertin¹  Ludovic Calandreau¹  Carole Moréno⁵  Patrice Dehais⁴  Sophie Leroux⁵  Cécile Arnould¹  Frédérique Pitel⁵  Julien Recoquillay³ 

[1] IFCE, Nouzilly, F-37380, France;INRA, UMR1313 GABI Génétique Animale et Biologie Intégrative, Jouy-en-Josas, F-78530, France;INRA, UR83 Recherches Avicoles, Nouzilly, F-37380, France;INRA, Sigenae UR875 Biométrie et Intelligence Artificielle, Castanet-Tolosan, F-31326, France;INPT ENVT Génétique Physiologie et Systèmes d’Elevage, INRA, Castanet-Tolosan, F-31326, France;UE1295 Pôle d’Expérimentation Avicole de Tours, Nouzilly, F-37380, France

Others  :  1109883
DOI  :  10.1186/s12864-014-1210-9

 received in 2014-07-18, accepted in 2014-12-30,  发布年份 2015

【 摘 要 】

Background

Behavioral traits such as sociability, emotional reactivity and aggressiveness are major factors in animal adaptation to breeding conditions. In order to investigate the genetic control of these traits as well as their relationships with production traits, a study was undertaken on a large second generation cross (F2) between two lines of Japanese Quail divergently selected on their social reinstatement behavior. All the birds were measured for several social behaviors (social reinstatement, response to social isolation, sexual motivation, aggression), behaviors measuring the emotional reactivity of the birds (reaction to an unknown object, tonic immobility reaction), and production traits (body weight and egg production).

Results

We report the results of the first genome-wide QTL detection based on a medium density SNP panel obtained from whole genome sequencing of a pool of individuals from each divergent line. A genetic map was constructed using 2145 markers among which 1479 could be positioned on 28 different linkage groups. The sex-averaged linkage map spanned a total of 3057 cM with an average marker spacing of 2.1 cM. With the exception of a few regions, the marker order was the same in Japanese Quail and the chicken, which confirmed a well conserved synteny between the two species. The linkage analyses performed using QTLMAP software revealed a total of 45 QTLs related either to behavioral (23) or production (22) traits. The most numerous QTLs (15) concerned social motivation traits. Interestingly, our results pinpointed putative pleiotropic regions which controlled emotional reactivity and body-weight of birds (on CJA5 and CJA8) or their social motivation and the onset of egg laying (on CJA19).

Conclusion

This study identified several QTL regions for social and emotional behaviors in the Quail. Further research will be needed to refine the QTL and confirm or refute the role of candidate genes, which were suggested by bioinformatics analysis. It can be hoped that the identification of genes and polymorphisms related to behavioral traits in the quail will have further applications for other poultry species (especially the chicken) and will contribute to solving animal welfare issues in poultry production.

【 授权许可】

   
2015 Recoquillay et al.; licensee Biomed Central.

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