Comparative genomics of Riemerella anatipestifer reveals genetic diversity

BMC Genomics

Xiaoyue Chen³  Anchun Cheng³  KunFeng Sun³  Shun Chen³  RenYong Jia³  MingShu Wang³  Sanjun Yin²  MaFeng Liu³  LinFeng Yang²  Dekang Zhu³  Wenbin Liu²  Xiaojia Wang¹ 

[1] Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China;BGI-Shenzhen, Shenzhen 518083, China;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China

关键词: Structural variation;    Comparative genomics;    Riemerella anatipestifer;   

Others  :  1089782
DOI  :  10.1186/1471-2164-15-479

 received in 2013-10-31, accepted in 2014-06-10,  发布年份 2014

【 摘 要 】

Background

Riemerella anatipestifer is one of the most important pathogens of ducks. However, the molecular mechanisms of R. anatipestifer infection are poorly understood. In particular, the lack of genomic information from a variety of R. anatipestifer strains has proved severely limiting.

Results

In this study, we present the complete genomes of two R. anatipestifer strains, RA-CH-1 (2,309,519 bp, Genbank accession CP003787) and RA-CH-2 (2,166,321 bp, Genbank accession CP004020). Both strains are from isolates taken from two different sick ducks in the SiChuang province of China. A comparative genomics approach was used to identify similarities and key differences between RA-CH-1 and RA-CH-2 and the previously sequenced strain RA-GD, a clinical isolate from GuangDong, China, and ATCC11845.

Conclusion

The genomes of RA-CH-2 and RA-GD were extremely similar, while RA-CH-1 was significantly different than ATCC11845. RA-CH-1 is 140,000 bp larger than the three other strains and has 16 unique gene families. Evolutionary analysis shows that RA-CH-1 and RA-CH-2 are closed and in a branch with ATCC11845, while RA-GD is located in another branch. Additionally, the detection of several iron/heme-transport related proteins and motility mechanisms will be useful in elucidating factors important in pathogenicity. This information will allow a better understanding of the phenotype of different R. anatipestifer strains and molecular mechanisms of infection.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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