Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment

BMC Microbiology

Alexandre S Rosado³  Regina MCP Domingues²  Raquel S Peixoto³  Casey RJ Hubert¹  Michael J Maguire¹  Geraldo R Paula²  Lívia Q Ferreira²  Edir M Ferreira³  Deborah CA Leite³  Luiza L Andrade¹ 

[1] School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, UK;Laboratório de Biologia de Anaeróbios, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Laboratório de Ecologia Molecular Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

关键词: Hydrocarbons;    Petroleum;    Sulphate;    Anoxic sediment;    Bacterial diversity;    Mangrove;   

Others  :  1221778
DOI  :  10.1186/1471-2180-12-186

 received in 2012-01-29, accepted in 2012-08-23,  发布年份 2012

【 摘 要 】

Background

Mangrove forests are coastal wetlands that provide vital ecosystem services and serve as barriers against natural disasters like tsunamis, hurricanes and tropical storms. Mangroves harbour a large diversity of organisms, including microorganisms with important roles in nutrient cycling and availability. Due to tidal influence, mangroves are sites where crude oil from spills farther away can accumulate. The relationship between mangrove bacterial diversity and oil degradation in mangrove sediments remains poorly understood.

Results

Mangrove sediment was sampled from 0–5, 15–20 and 35–40 cm depth intervals from the Suruí River mangrove (Rio de Janeiro, Brazil), which has a history of oil contamination. DGGE fingerprinting for bamA, dsr and 16S rRNA encoding fragment genes, and qPCR analysis using dsr and 16S rRNA gene fragment revealed differences with sediment depth.

Conclusions

Analysis of bacterial 16S rRNA gene diversity revealed changes with depth. DGGE for bamA and dsr genes shows that the anaerobic hydrocarbon-degrading community profile also changed between 5 and 15 cm depth, and is similar in the two deeper sediments, indicating that below 15 cm the anaerobic hydrocarbon-degrading community appears to be well established and homogeneous in this mangrove sediment. qPCR analysis revealed differences with sediment depth, with general bacterial abundance in the top layer (0–5 cm) being greater than in both deeper sediment layers (15–20 and 35–40 cm), which were similar to each other.

【 授权许可】

   
2012 Andrade et al.; licensee BioMed Central Ltd.

【 图 表 】

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