期刊论文详细信息
BMC Complementary and Alternative Medicine
Antioxidant and antidiabetic profiles of two African medicinal plants: Picralima nitida (Apocynaceae) and Sonchus oleraceus (Asteraceae)
Fabrice Fekam Boyom2  Bruno Tugnoua Tchinda3  Denis Zofou1  Pascaline Chouadeu Mejiato3  Clautilde Mofor Teugwa3 
[1] Biotechnology Unit, University of Buea, Buea, South West Region, Cameroon;Laboratoire de Phytoprotection et de valorisation des resources végétales, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Biotechnology Centre, Yaoundé, Cameroon;Laboratory of Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box: 812, Yaoundé, Cameroon
关键词: Pricralima nitida;    Sonchus oleraceus;    Hypoglycaemic activity;    Oxidative stress;    Diabetes;    Antioxidant;   
Others  :  1221071
DOI  :  10.1186/1472-6882-13-175
 received in 2013-01-23, accepted in 2013-07-11,  发布年份 2013
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【 摘 要 】

Background

Diabetes mellitus (DM) is a metabolic disease characterized by chronic hyperglycaemia generally associated with oxidative stress. The present study aims at evaluating the antioxidant and antidiabetic potential of methanol and hydroethanol extracts of the stem bark and leaves of Pricralima nitida and the Sonchus oleraceus whole plant respectively.

Methods

The in vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH) for free radical-scavenging properties of the extracts, and the Folin-Ciocalteu method in determining their phenol contents. The antidiabetic activity was tested in mice following streptozotocin diabetes induction, and selected oxidative stress markers (Malondialdehyde, Hydrogen peroxides and Catalase) were measured in order to evaluate the level of oxidative stress in treated animals.

Results

The in vitro antioxidant activity using DPPH showed IC50 ranging from 0.19 ± 0.08 to 1.00 ± 0.06 mg/mL. The highest activity was obtained with the hydroethanol extracts of S. oleraceus (0.19 mg/mL and P. nitida (0.24 mg/mL). Polyphenol contents ranged from 182.25 ± 16.76 to 684.62 ± 46.66 μg Eq Cat/g. The methanol extract of P. nitida showed the highest activity, followed by the hydroethanol extract of S. oleraceus (616.89 ± 19.20 μEq Cat/g). The hydroethanol extract of whole plants (150 mg/Kg) and methanol leave extract of P. nitida (300 mg/Kg) exhibited significant antidiabetic activities with 39.40% and 38.48% glycaemia reduction, respectively. The measurement of stress markers in plasma, liver and kidney after administration of both extracts showed significant reduction in MDA and hydrogen peroxide levels, coupled with a substantial increase in catalase activity.

Conclusions

These findings suggest that S. oleraceus whole plant and P. nitida leaves possess both antidiabetic and antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules against diabetes.

【 授权许可】

   
2013 Teugwa et al.; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]Valiathan MS: Healing plants. Curr Sci 1998, 75:1122-1127.
  • [2]Collier A, Wilson R, Bradley H, Thomson JA, Small M: Free radical activity in type 2 diabetes. Diabetes Med 1990, 7:27-30.
  • [3]Braynes JW: Role of oxidative stress in development of complications in diabetes. Diabetes 1991, 40:405-412.
  • [4]Dieye AM, Sarr A, Diop SN, N’Diaye M, Sy GY, Diarra M, Gaffary LR, Sy AN, Faye B: Medicinal plants and the treatment of diabetes in Senegal: Survey with patients. Fundamental Clin Pharmacol 2008, 22:211-216.
  • [5]Mbanya JCN, Motala AA, Sobngwi E, Assah FK, Enoru ST: Diabetes in sub-Saharan Africa. Lancet 2010, 375:2254-2266.
  • [6]Babu V, Gangadevi T, Subramoniam A: Anti-hyperglycaemic activity of cassia kleinii leaf extract in glucose fed normal rats and alloxan-induced diabetic rats. Indian J Pharmacol 2002, 34:409-415.
  • [7]N’guessan K, Tiebre M, Ake-Assi E, Zirihi G: Ethnobotanical study of plants used to treat arterial hypertension in Traditional Medicine, by Abbey and Krobou populations of Agboville (Côte-d’Ivoire). European J Sci Res 2009, 35:85-98.
  • [8]Inya-Agha SI, Ezea SC, Odukoya OA: Evaluation of Picralima nitida hypoglycaemic activity, toxicity and analytical standards. Planta Med 2006, 10:551.
  • [9]Okonta JM, Aguwa CN: Evaluation of Hypoglycemic Activity of Glycosides and Alkaloids Extracts of Picralima nitida Stapf (Apocynaceae) Seed. Int J Pharmacol 2007, 3(6):505-509.
  • [10]Odebiyi O, Sofowora EA: Phytochemical screening: Nigeria Medicinal plants. Loydia 1978, 41:234-235.
  • [11]Hotano T, Kasuhara T, Okuda T: Two new flavonoids and other constituants in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull 1988, 36:2090-2097.
  • [12]Molyneux P: The use of stable free radical Diphenyl Picryl Hydrazyl (DPPH) for establishing antioxidant activity. Songklamakarin J Sci Tech 2004, 26:211-219.
  • [13]Singleton VL, Rossi JA: Colorimetry of total phenolics with phosphomolybdic phosphotungstic reagents acid. Am J Ecol Viticulture 1965, 16:144-158.
  • [14]Olivier D, Aoubault E, Pinatel C, Souilol S, Guerese M, Artaud J: Analyse de la fraction phenolique des huiles d’olive vierges. Annal Expert Forum Chem Toxicol 2004, 965:169-196.
  • [15]Al-Shamaony LA, Al-Khazraji SM, Twaiji HAA: Hypoglycemic effect of Artemisia herba alba II: effect of a valuable extract on some blood parameters in diabetic animals. J Ethnopharmacol 1994, 43:167-171.
  • [16]Yagi K: Simple Fluorometric Assay for lipoperoxyde in blood plasma. Biochem Med 1976, 8:950-988.
  • [17]Jiang ZY, Hunt JV, Wolft SD: Ferrous ion in oxidation in the presence of xylenol orange for detection of lipid hydroxide in low density lipoprotein. Anal Biochem 1992, 202:384-389.
  • [18]Sinha KA: Colorimetric assay of catalase. Anal Biochem 1972, 47(2):389-394.
  • [19]Aronoff SL, Berkowitz K, Barb Shreiner B: Want L. Glucose Metabolism and Regulation: Beyond Insulin and Glucagon. Diabetes Spectrum 2004, 17(3):183-190.
  • [20]Stanley MPP, Kamalakkannan N, Menon PV: Antidiabetic and antihyperlipidemic effect of alcoholic Syzigium cumini seeds in alloxan induced diabetic albino rats. J Ethnopharmacol 2004, 91:209-213.
  • [21]Shetty K, Wahlqvist ML: A model for the role of the proline-linked pentose-phosphate pathway in phenolic phytochemical bio-synthesis and mechanism of action for human health and environmental applications. Asia Pac J Clin Nutr 2004, 13:1-24.
  • [22]Akinmoladun AC, Ibuku EO, Dan-Ologe IA: Phytochemical constituents and antioxidatant properties of extracts from leaves of Chromolaena odorata. Sci Res Essays 2007, 2(6):537-544.
  • [23]Ivora MD, Paya M, Villar A: A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol 1989, 27:243-275.
  • [24]Ikarashi N, Toda T, Okaniwa T, Ito K, Ochiai W, Sugiyama K: Anti-Obesity and Anti-Diabetic Effects of Acacia Polyphenol in Obese Diabetic KKAy Mice Fed High-Fat Diet. Evid Based Complement Alternat Med 2011, 2011:952031. Epub 2011 Apr 14
  • [25]Kakkar R, Kalra J, Mantha SV, Prasad K: Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem 1995, 151:113-119.
  • [26]Davi G, Falco A, Patrono C: Lipid peroxidation in diabetes mellitus. Antioxid Redox Signal 2005, 7:256-268.
  • [27]Opara EC: Oxidative stress, micronutrients, diabetes mellitus and its complications. J R Soc Promot Health 2002, 122:28-34.
  • [28]Hunt JV, Smith CC, Wolff SP: Autoxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose. Diabetes 1990, 39:1420-1424.
  • [29]Pavana P, Sethupathy S, Manoharan S: Antihyperglycemic and antiproliferative effects of Tephrosia purpurea seed extract in streptozotocin induced diabetic rats. Indian J Clin Biochem 2007, 22(1):77-83.
  • [30]Nwakile CD, Okore VC: Picralima Nitida Seed Oil I: Hypoglycemic Activity. J Adv Pharm Educ Res 2011, 2:147-150.
  • [31]Ahmed OM, Hozayen WGM, Bastawy M, Hamed MZ: Biochemical Effects of Cichorium intybus and Sonchus oleraceus Infusions and Esculetin on Streptozotocin-Induced Diabetic Albino Rats. J Am Sci 2011, 7(12):1124-1137.
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