Antinociceptive effect of methanol extract of leaves of Persicaria hydropiper in mice

BMC Complementary and Alternative Medicine

Md Sohel Rana²  Mohammad Zafar Imam¹  Ambia Khatun¹ 

[1]Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka, 1217, Bangladesh

[2]Department of Pharmacy, Jahangirnagar University, Dhaka, 1342, Savar, Bangladesh

关键词: Opoid system;    Pain;    Antinociceptive;    Polygonaceae;    Persicaria hydropiper;   

Others  :  1137763
DOI  :  10.1186/s12906-015-0558-y

 received in 2014-10-06, accepted in 2015-02-18,  发布年份 2015

【 摘 要 】

Background

Persicaria hydropiper (Linn.) Delarbre is a common plant of Polygonaceae family commonly called Bishkatali in Bangladesh. Leaves of the plant are traditionally used in the treatment of rheumatic pain, gout, and skin diseases such as ringworms, scabies, boils, abscesses, carbuncles, bites of snakes, dogs or insects. This study evaluated the antinociceptive effect of the methanol extract of P. hydropiper leaves (MEPH).

Methods

The antinociceptive activity of MEPH was investigated using heat-induced (hot-plate and tail-immersion test) and chemical-induced (acetic acid, formalin, glutamic acid, cinnamaldehyde) nociception models in mice at 25, 50, and 75 mg/kg doses. Involvement of opioid system, cyclic guanosine monophosphate (cGMP) pathway, and ATP-sensitive K⁺ channel pathway were also tested using naloxone, methylene blue and glibenclamide respectively.

Results

MEPH showed antinociceptive activity in both heat- and chemical induced pain models. In both hot plate and tail immersion tests MEPH significantly increases the latency to the thermal stimuli. In acetic acid-induced writhing test the extract inhibited the number of abdominal writhing. Likewise, MEPH produced significant dose-dependent inhibition of paw licking in both neurogenic and inflammatory pain induced by intraplantar injection of formalin. Besides, MEPH also significantly inhibited the glutamate-induced pain and cinnamaldehyde-induced pain in mice. It was also clear that pretreatment with naloxone significantly reversed the antinociception produced by MEPH in hot plate and tail immersion test suggesting the involvement of opioid system in its effect. In addition, administration of methylene blue, a non specific inhibitor of NO/guanylyl cyclase, enhanced MEPH induced antinociception while glibenclamide, an ATP-sensitive K⁺ channel antagonist, could not reverse antinociceptive activity induced by MEPH.

Conclusion

Based on the results of the current study it can be said that MEPH possesses significant antinociceptive activity which acts in both peripheral and central mechanisms.

【 授权许可】

   
2015 Khatun et al.; licensee BioMed Central.

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