Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells

BMC Microbiology

Tânia A T Gomes²  Ana Flavia Popi²  Lika Osugui²  Denise Yamamoto²  Rodrigo T Hernandes¹  Fabiano T Romão² 

[1] Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil;Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina, São Paulo, SP, Brazil

关键词: Diarrhea;    Environmental factors;    Adherence;    type I pilus;    Glucose;    Atypical EPEC;   

Others  :  1090536
DOI  :  10.1186/s12866-014-0299-y

 received in 2014-07-15, accepted in 2014-11-18,  发布年份 2014

【 摘 要 】

Background

Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO₂ (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551–2.

Results

We found that a CO₂-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551–2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551–2 strain to HeLa cells.

Conclusions

Our data suggest that a CO₂-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process.

【 授权许可】

   
2015 Romão et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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