Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes

BMC Genomics

Emanuel M Souza²  Fábio O Pedrosa²  Leda S Chubatsu²  Vinicius A Weiss²  Helisson Faoro²  Lucélia Donatti¹  Glaucio Valdameri²  Liziane CC Brusamarello-Santos²  Michelle Z Tadra-Sfeir²  Roseli Wassem⁴  Paloma Bonato²  Doumit Camilios-Neto³ 

[1] Department of Cellular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil;Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR 81531-990, Brazil;Departament of Biochemistry and Biotechnology, Universidade Estadual de Londrina, Londrina, PR, Brazil;Department of Genetics, Universidade Federal do Paraná, Curitiba, PR, Brazil

关键词: Cell cycle and nitrogen fixation;    PGPB;    Azospirilum brasilense;    Triticum aestivum;    Wheat;    Transcriptional analysis;    RNA-seq;   

Others  :  1217223
DOI  :  10.1186/1471-2164-15-378

 received in 2013-11-15, accepted in 2014-05-02,  发布年份 2014

【 摘 要 】

Background

The rapid growth of the world’s population demands an increase in food production that no longer can be reached by increasing amounts of nitrogenous fertilizers. Plant growth promoting bacteria (PGPB) might be an alternative to increase nitrogenous use efficiency (NUE) in important crops such wheat. Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by A. brasilense is a good model to investigate the molecular basis of plant-PGPB interaction including improvement in plant-NUE promoted by PGPB.

Results

We performed a dual RNA-Seq transcriptional profiling of wheat roots colonized by A. brasilense strain FP2. cDNA libraries from biological replicates of colonized and non-inoculated wheat roots were sequenced and mapped to wheat and A. brasilense reference sequences. The unmapped reads were assembled de novo. Overall, we identified 23,215 wheat expressed ESTs and 702 A. brasilense expressed transcripts. Bacterial colonization caused changes in the expression of 776 wheat ESTs belonging to various functional categories, ranging from transport activity to biological regulation as well as defense mechanism, production of phytohormones and phytochemicals. In addition, genes encoding proteins related to bacterial chemotaxi, biofilm formation and nitrogen fixation were highly expressed in the sub-set of A. brasilense expressed genes.

Conclusions

PGPB colonization enhanced the expression of plant genes related to nutrient up-take, nitrogen assimilation, DNA replication and regulation of cell division, which is consistent with a higher proportion of colonized root cells in the S-phase. Our data support the use of PGPB as an alternative to improve nutrient acquisition in important crops such as wheat, enhancing plant productivity and sustainability.

【 授权许可】

   
2014 Camilios-Neto et al.; licensee BioMed Central Ltd.

【 图 表 】

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