The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide

BMC Research Notes

John Barrett²  James Brennan²  Thomas Patton²  Matthew Dryden¹  Jonathan Cooke³ 

[1] Rare and imported pathogens laboratory (RIPL), Public Health England, Manor Farm Road, Porton Down, Wiltshire SP4 0JG, UK;Institute of Technology, Ash Lane, Sligo, Ireland;Manchester Pharmacy School, Faculty of Medical and Human Sciences University of Manchester, Manchester, UK

关键词: Wound dressings;    Antimicrobial;    Hydrogen peroxide;    Modified honey;    Honey;    Reactive oxygen species (ROS);   

Others  :  1109642
DOI  :  10.1186/s13104-014-0960-4

 received in 2014-04-29, accepted in 2014-12-23,  发布年份 2015

【 摘 要 】

Background

Antimicrobial resistance continues to be a global issue in healthcare organisations. Honey has long been shown to possess wound healing and antimicrobial properties that are dependent on a number of physical and chemical properties of the honey. We tested the antimicrobial activity of a medicinal honey, Surgihoney® (SH) and two prototype modified honeys made by Apis mellifera (honeybee) against Staphylococcus aureus (NCIMB 9518). We also examined the modified honey prototypes for the ability to generate reactive oxygen species (ROS) by changing the level of production of hydrogen peroxide from the samples.

Methods

Surgihoney® (SH) was compared with two modified honeys, Prototype 1 (PT1) and Prototype 2 (PT2) using a bioassay method against a standard strain of Staphylococcus aureus. Further work studied the rate of generation of ROS hydrogen peroxide from these preparations.

Results

Surgihoney® antimicrobial activity was shown to be largely due to ROS hydrogen peroxide production. By modification of Surgihoney®, two more potent honey prototypes were shown to generate between a two- and three-fold greater antibacterial activity and up to ten times greater ROS peroxide activity.

Conclusions

Surgihoney® is a clinically available wound antiseptic dressing that shows good antimicrobial activity. Two further honey prototypes have been shown to have antimicrobial activity that is possible to be enhanced due to demonstrated increases in ROS peroxide activity.

【 授权许可】

   
2015 Cooke et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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